Calibration Curve: EAQLPVIENK: /Panorama Public/2024/MRM Proteomics

Expected Concentration	1.0	Bias (%)	2.5	Bias (%)	5.0	Bias (%)	20.0	Bias (%)	40.0	Bias (%)	160.0	Bias (%)	400.0	Bias (%)	1000.0	Bias (%)
Calculated Concentrations	52.81	5181.17	118.33	4633.31	225.88	4417.52	1003.34	4916.72	1984.84	4862.10	8746.64	5366.65	19572.46	4793.12	55143.02	5414.30

n	1		1		1		1		1		1		1		1
Mean	52.81		118.33		225.88		1003.34		1984.84		8746.64		19572.46		55143.02
StdDev	NA		NA		NA		NA		NA		NA		NA		NA
CV(%)	NA		NA		NA		NA		NA		NA		NA		NA
Bias(%)	5181.17		4633.31		4417.52		4916.72		4862.10		5366.65		4793.12		5414.30

Expected Concentration	4.0	Bias (%)	30.0	Bias (%)	300.0	Bias (%)
Calculated Concentrations	161.88	3947.06	1386.04	4520.12	15132.62	4944.21
	170.89	4172.35	1393.37	4544.56	15090.94	4930.31
	164.83	4020.67	1377.24	4490.80	15178.41	4959.47

n	3		3		3
Mean	165.87		1385.55		15133.99
StdDev	4.59		8.07		43.75
CV(%)	2.77		0.58		0.29
Bias(%)	4046.69		4518.49		4944.66

Sample File Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity L Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity H Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	L/H Ratio Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Exp Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Measured Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
Sample File Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity L Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity H Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	L/H Ratio Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Exp Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Measured Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
20220516_PC5_ClientDigest	2+	EAQLPVIENK	16.2712	48,703.6484	418,191.4688	0.12		791.942
20220516_PC5_neat_R01	2+	EAQLPVIENK	16.0058	220,308.6562	2,036,927.0000	0.11		736.611
20220516_PC5_neat_R02B	2+	EAQLPVIENK	16.0710	562,623.5000	4,989,614.5000	0.11		767.268
20220516_PC5_neat_R03	2+	EAQLPVIENK	16.2124	260,796.7812	2,055,055.6250	0.13		861.513
20220516_Seer_NP01_R01	2+	EAQLPVIENK	16.1136	1,287,627.3750	826,252.3125	1.56		10398.575
20220516_Seer_NP01_R02	2+	EAQLPVIENK	16.1038	1,419,729.3750	796,537.8125	1.78		11890.807
20220516_Seer_NP01_R03	2+	EAQLPVIENK	16.1136	1,536,693.6250	812,352.5625	1.89		12618.888
20220516_Seer_NP01_R04	2+	EAQLPVIENK	16.1136	1,376,468.6250	791,625.9375	1.74		11600.405
20220516_Seer_NP01_R05	2+	EAQLPVIENK	16.1136	1,299,940.0000	497,518.3438	2.61		17423.695
20220516_Seer_NP02_R01	2+	EAQLPVIENK	16.1136	127,863.7266	703,782.4375	0.18		1226.448
20220516_Seer_NP02_R02	2+	EAQLPVIENK	16.1234	103,168.1641	662,303.4375	0.16		1053.833
20220516_Seer_NP02_R03	2+	EAQLPVIENK	16.1234	97,037.2109	689,074.3125	0.14		954.237
20220516_Seer_NP02_R04	2+	EAQLPVIENK	16.1333	98,077.6562	664,178.0625	0.15		999.842
20220516_Seer_NP02_R05	2+	EAQLPVIENK	16.1332	89,135.0391	610,522.3125	0.15		988.717
20220516_Seer_NP03_R01	2+	EAQLPVIENK	16.1136	547,836.7500	808,382.5000	0.68		4531.059
20220516_Seer_NP03_R02	2+	EAQLPVIENK	16.1234	508,740.2188	673,454.6875	0.76		5048.880
20220516_Seer_NP03_R03	2+	EAQLPVIENK	16.1332	487,485.6562	667,882.5000	0.73		4878.849
20220516_Seer_NP03_R04	2+	EAQLPVIENK	16.1235	541,898.7500	696,114.9375	0.78		5202.400
20220516_Seer_NP03_R05	2+	EAQLPVIENK	16.1332	451,535.2812	496,767.6875	0.91		6071.734
20220516_Seer_NP04_R01	2+	EAQLPVIENK	16.1332	6,103.7144	781,802.5000	0.01		68.045
20220516_Seer_NP04_R02	2+	EAQLPVIENK	16.1430	3,621.2776	741,370.0000	0.00		48.573
20220516_Seer_NP04_R03	2+	EAQLPVIENK	16.1430	4,756.4595	782,929.2500	0.01		56.505
20220516_Seer_NP04_R04	2+	EAQLPVIENK	16.1332	4,993.7617	950,069.6250	0.01		51.049
20220516_Seer_NP04_R05	2+	EAQLPVIENK	16.1822	5,577.5762	623,090.0000	0.01		75.668
20220516_Seer_NP05_R01	2+	EAQLPVIENK	16.1430	1,996,100.3750	899,151.2500	2.22		14806.294
20220516_Seer_NP05_R02	2+	EAQLPVIENK	16.1528	2,269,448.7500	998,128.7500	2.27		15164.198
20220516_Seer_NP05_R03	2+	EAQLPVIENK	16.1635	1,881,078.7500	810,280.3125	2.32		15482.742
20220516_Seer_NP05_R04	2+	EAQLPVIENK	16.1341	1,862,185.1250	937,636.2500	1.99		13247.703
20220516_Seer_NP05_R05	2+	EAQLPVIENK	16.1831	1,255,449.3750	514,756.7812	2.44		16264.910
20220518_Seer_QC-A_R01	2+	EAQLPVIENK	16.2614	84,015.0703	3,837,694.0000	0.02	4.0	161.882
20220518_Seer_QC-A_R02	2+	EAQLPVIENK	16.2799	83,415.9219	3,588,606.2500	0.02	4.0	170.894
20220518_Seer_QC-A_R03	2+	EAQLPVIENK	16.2604	79,578.5000	3,563,105.5000	0.02	4.0	164.827
20220518_Seer_QC-B_R01	2+	EAQLPVIENK	16.3104	754,577.7500	3,669,506.0000	0.21	30.0	1386.037
20220518_Seer_QC-B_R02	2+	EAQLPVIENK	16.2800	711,675.5625	3,442,454.5000	0.21	30.0	1393.367
20220518_Seer_QC-B_R03	2+	EAQLPVIENK	16.2799	711,321.2500	3,481,503.7500	0.20	30.0	1377.240
20220518_Seer_QC-C_R01	2+	EAQLPVIENK	16.2908	7,931,735.0000	3,495,752.7500	2.27	300.0	15132.619
20220518_Seer_QC-C_R02	2+	EAQLPVIENK	16.3192	7,519,019.0000	3,323,019.2500	2.26	300.0	15090.937
20220518_Seer_QC-C_R03	2+	EAQLPVIENK	16.2995	7,597,153.5000	3,338,181.0000	2.28	300.0	15178.408
20220518_Seer_Std_A	2+	EAQLPVIENK	16.2712	21,412.8223	3,878,556.2500	0.01	1.0	52.812
20220518_Seer_Std_B	2+	EAQLPVIENK	16.2516	61,939.3086	4,033,722.0000	0.02	2.5	118.333
20220518_Seer_Std_C	2+	EAQLPVIENK	16.2908	117,970.4219	3,745,409.5000	0.03	5.0	225.876
20220518_Seer_Std_D	2+	EAQLPVIENK	16.2614	559,784.7500	3,777,395.5000	0.15	20.0	1003.343
20220518_Seer_Std_E	2+	EAQLPVIENK	16.2516	1,127,073.8750	3,813,949.0000	0.30	40.0	1984.840
20220518_Seer_Std_F	2+	EAQLPVIENK	16.3006	4,195,700.0000	3,201,741.0000	1.31	160.0	8746.639
20220518_Seer_Std_G	2+	EAQLPVIENK	16.2810	9,333,645.0000	3,179,713.5000	2.94	400.0	19572.461
20220518_Seer_Std_H	2+	EAQLPVIENK	16.3094	20,712,838.0000	2,503,235.2500	8.27	1000.0	55143.015

Lower Limit of Detection	NA
Lower Limit of Quantitation	NA
Upper Limit of Quantitation	NA
LOQ Bias Limit	30.0%
LOQ CV Limit	N/A
LOD Calculation	None
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Calibration Curve: EAQLPVIENK

Calibration Curve

Figures of Merit

Concentrations in Standards

Concentrations in Quality Control

Blanks

Summary Charts

Peak Areas

Retention Times

Quantitation Ratios