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  1. Targeted MS Runs
  2. S-0536_H_sap_pano_2024-07-05_11-19-11.sky.zip

Calibration Curve: AVEPQLQEEER

MRM Proteomics - Seer Proteograph

Calibration Curve

 
  
050010001500200025003000350040002468101214AVEPQLQEEERAnalyte Concentration Normalized Peak AreasName: 8_Seer_QC-A_R01 Peak Area: 0.03545 Concentration: 14.53036Name: 8_Seer_QC-A_R02 Peak Area: 0.04224 Concentration: 14.53036Name: 8_Seer_QC-A_R03 Peak Area: 0.03898 Concentration: 14.53036Name: 8_Seer_QC-B_R01 Peak Area: 0.30743 Concentration: 108.97769Name: 8_Seer_QC-B_R02 Peak Area: 0.31525 Concentration: 108.97769Name: 8_Seer_QC-B_R03 Peak Area: 0.31777 Concentration: 108.97769Name: 8_Seer_QC-C_R01 Peak Area: 3.80392 Concentration: 1089.77688Name: 8_Seer_QC-C_R02 Peak Area: 3.8245 Concentration: 1089.77688Name: 8_Seer_QC-C_R03 Peak Area: 3.68957 Concentration: 1089.77688Name: 8_Seer_Std_A Peak Area: 0.0114 Concentration: 3.63259 Excluded from calibrationName: 8_Seer_Std_B Peak Area: 0.02046 Concentration: 9.08147Name: 8_Seer_Std_C Peak Area: 0.05026 Concentration: 18.16295Name: 8_Seer_Std_D Peak Area: 0.20802 Concentration: 72.65179Name: 8_Seer_Std_E Peak Area: 0.44542 Concentration: 145.30358Name: 8_Seer_Std_F Peak Area: 2.17513 Concentration: 581.21433Name: 8_Seer_Std_G Peak Area: 4.91712 Concentration: 1453.03584Name: 8_Seer_Std_H Peak Area: 14.27112 Concentration: 3632.58959Name: 6_Seer_NP01_R01 Peak Area: 0.11316 Concentration: 36.10319Name: 6_Seer_NP01_R02 Peak Area: 0.11308 Concentration: 36.08087Name: 6_Seer_NP01_R03 Peak Area: 0.10729 Concentration: 34.40891Name: 6_Seer_NP01_R04 Peak Area: 0.11775 Concentration: 37.43068Name: 6_Seer_NP01_R05 Peak Area: 0.16362 Concentration: 50.6823Name: 6_Seer_NP02_R01 Peak Area: 0.15469 Concentration: 48.10452Name: 6_Seer_NP02_R02 Peak Area: 0.16109 Concentration: 49.95381Name: 6_Seer_NP02_R03 Peak Area: 0.15624 Concentration: 48.55135Name: 6_Seer_NP02_R04 Peak Area: 0.13345 Concentration: 41.96556Name: 6_Seer_NP02_R05 Peak Area: 0.15423 Concentration: 47.96938Name: 6_Seer_NP03_R01 Peak Area: 0.07986 Concentration: 26.48136Name: 6_Seer_NP03_R02 Peak Area: 0.06174 Concentration: 21.2483Name: 6_Seer_NP03_R03 Peak Area: 0.04972 Concentration: 17.77341Name: 6_Seer_NP03_R04 Peak Area: 0.0669 Concentration: 22.73794Name: 6_Seer_NP03_R05 Peak Area: 0.06597 Concentration: 22.47089Name: 6_Seer_NP04_R01 Peak Area: 0.13962 Concentration: 43.74877Name: 6_Seer_NP04_R02 Peak Area: 0.10781 Concentration: 34.55691Name: 6_Seer_NP04_R03 Peak Area: 0.08063 Concentration: 26.70608Name: 6_Seer_NP04_R04 Peak Area: 0.07246 Concentration: 24.34401Name: 6_Seer_NP04_R05 Peak Area: 0.1163 Concentration: 37.00998Name: 6_Seer_NP05_R01 Peak Area: 0.13206 Concentration: 41.56517Name: 6_Seer_NP05_R02 Peak Area: 0.12483 Concentration: 39.47569Name: 6_Seer_NP05_R03 Peak Area: 0.1226 Concentration: 38.83272Name: 6_Seer_NP05_R04 Peak Area: 0.12145 Concentration: 38.50036Name: 6_Seer_NP05_R05 Peak Area: 0.15457 Concentration: 48.06718Name: 6_PC5_neat_R01 Peak Area: 0.05456 Concentration: 19.17357Name: 6_PC5_neat_R02B Peak Area: 0.05686 Concentration: 19.83683Name: 6_PC5_neat_R03 Peak Area: 0.06157 Concentration: 21.19795Name: 6_PC5_ClientDigest Peak Area: 0.16702 Concentration: 51.667StandardQCUnknownExcludedCalibration CurveRegression Fit: linearNorm. Method: ratio_to_heavyRegression Weighting: 1/(x*x)MS Level: AllSlope: 0.00346Intercept: -0.0118rSquared: 0.9818

Figures of Merit

 
Lower Limit of Detection NA
Lower Limit of Quantitation NA
Upper Limit of Quantitation NA
LOQ Bias Limit 30.0%
LOQ CV Limit N/A
LOD Calculation None
Show Details

Summary Charts

 

Peak Areas

AVEPQLQEEER
++ light++ heavy8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_Std_F8_Seer_S...8_Seer_S...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_PC5_ne...6_PC5_ne...6_Seer_N...6_PC5_ne...6_PC5_Cli...0100200300400500600700Peak Area 10^3
Replicates

Retention Times

AVEPQLQEEER
++ light++ heavy8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_S...8_Seer_Std_F8_Seer_S...8_Seer_S...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_Seer_N...6_PC5_ne...6_PC5_ne...6_Seer_N...6_PC5_ne...6_PC5_Cli...8.68.78.88.99.09.19.2Retention Time
Replicates

Quantitation Ratios

 
1 - 46 of 46
20220516_PC5_ClientDigest2+AVEPQLQEEER9.00811,009.19826,042.22560.17 51.667
20220516_PC5_neat_R012+AVEPQLQEEER8.77021,968.799336,083.28520.05 19.174
20220516_PC5_neat_R02B2+AVEPQLQEEER8.90085,796.2236101,941.72660.06 19.837
20220516_PC5_neat_R032+AVEPQLQEEER8.94872,301.599937,382.37110.06 21.198
20220516_Seer_NP01_R012+AVEPQLQEEER8.83951,647.933514,563.24510.11 36.103
20220516_Seer_NP01_R022+AVEPQLQEEER8.82961,588.621614,048.68070.11 36.081
20220516_Seer_NP01_R032+AVEPQLQEEER8.80981,478.677713,781.67770.11 34.409
20220516_Seer_NP01_R042+AVEPQLQEEER8.82961,597.303313,565.03320.12 37.431
20220516_Seer_NP01_R052+AVEPQLQEEER8.81971,449.98478,862.11230.16 50.682
20220516_Seer_NP02_R012+AVEPQLQEEER8.82961,948.743812,597.38480.15 48.105
20220516_Seer_NP02_R022+AVEPQLQEEER8.82961,865.208111,578.32420.16 49.954
20220516_Seer_NP02_R032+AVEPQLQEEER8.82961,925.350112,322.96390.16 48.551
20220516_Seer_NP02_R042+AVEPQLQEEER8.83951,540.439911,543.45700.13 41.966
20220516_Seer_NP02_R052+AVEPQLQEEER8.81971,509.45259,787.23930.15 47.969
20220516_Seer_NP03_R012+AVEPQLQEEER8.82961,233.756115,449.89160.08 26.481
20220516_Seer_NP03_R022+AVEPQLQEEER8.8296738.407211,959.29390.06 21.248
20220516_Seer_NP03_R032+AVEPQLQEEER8.8296575.603911,577.70210.05 17.773
20220516_Seer_NP03_R042+AVEPQLQEEER8.8197865.298012,934.37210.07 22.738
20220516_Seer_NP03_R052+AVEPQLQEEER8.8296569.31068,629.20800.07 22.471
20220516_Seer_NP04_R012+AVEPQLQEEER8.84941,649.349111,813.22950.14 43.749
20220516_Seer_NP04_R022+AVEPQLQEEER8.83951,315.173312,199.52930.11 34.557
20220516_Seer_NP04_R032+AVEPQLQEEER8.8494919.924511,408.77050.08 26.706
20220516_Seer_NP04_R042+AVEPQLQEEER8.85931,006.436913,889.96680.07 24.344
20220516_Seer_NP04_R052+AVEPQLQEEER8.88901,164.195710,010.67190.12 37.010
20220516_Seer_NP05_R012+AVEPQLQEEER8.88901,673.873212,674.97560.13 41.565
20220516_Seer_NP05_R022+AVEPQLQEEER8.87911,805.402814,462.95610.12 39.476
20220516_Seer_NP05_R032+AVEPQLQEEER8.91901,456.795711,882.11330.12 38.833
20220516_Seer_NP05_R042+AVEPQLQEEER8.86951,757.630414,471.59860.12 38.500
20220516_Seer_NP05_R052+AVEPQLQEEER8.90911,181.30467,642.76420.15 48.067
20220518_Seer_QC-A_R012+AVEPQLQEEER9.00812,465.749369,559.31250.044.013.651
20220518_Seer_QC-A_R022+AVEPQLQEEER9.04182,932.319669,417.71880.044.015.614
20220518_Seer_QC-A_R032+AVEPQLQEEER8.99232,618.025167,170.27340.044.014.670
20220518_Seer_QC-B_R012+AVEPQLQEEER9.047720,892.072367,958.14060.3130.092.233
20220518_Seer_QC-B_R022+AVEPQLQEEER9.031920,311.701264,429.78910.3230.094.495
20220518_Seer_QC-B_R032+AVEPQLQEEER9.002220,608.824264,854.72660.3230.095.222
20220518_Seer_QC-C_R012+AVEPQLQEEER9.0279249,404.421965,565.16413.80300.01102.471
20220518_Seer_QC-C_R022+AVEPQLQEEER9.0418242,827.625063,492.67583.82300.01108.417
20220518_Seer_QC-C_R032+AVEPQLQEEER9.0517243,179.421965,909.92973.69300.01069.433
20220518_Seer_Std_A2+AVEPQLQEEER9.0180823.975372,310.06250.011.06.701
20220518_Seer_Std_B2+AVEPQLQEEER8.97841,490.398672,848.26560.022.59.320
20220518_Seer_Std_C2+AVEPQLQEEER9.00813,391.041567,476.49220.055.017.929
20220518_Seer_Std_D2+AVEPQLQEEER9.008114,931.258871,779.61720.2120.063.511
20220518_Seer_Std_E2+AVEPQLQEEER8.998231,459.363370,628.12500.4540.0132.104
20220518_Seer_Std_F2+AVEPQLQEEER9.0477129,539.218859,554.74612.18160.0631.867
20220518_Seer_Std_G2+AVEPQLQEEER9.0081306,212.218862,274.76954.92400.01424.106
20220518_Seer_Std_H2+AVEPQLQEEER9.0418719,292.500050,401.976614.271000.04126.749