Calibration Curve: GFPSVLR: /Panorama Public/2024/MRM Proteomics

Expected Concentration	1.0	Bias (%)	2.5	Bias (%)	5.0	Bias (%)	20.0	Bias (%)	40.0	Bias (%)	160.0	Bias (%)	400.0	Bias (%)	1000.0	Bias (%)
Calculated Concentrations	38.33	3732.86	83.25	3229.80	164.18	3183.65	708.39	3441.97	1401.08	3402.71	6705.78	4091.11	15923.28	3880.82	34250.74	3325.07

n	1		1		1		1		1		1		1		1
Mean	38.33		83.25		164.18		708.39		1401.08		6705.78		15923.28		34250.74
StdDev	NA		NA		NA		NA		NA		NA		NA		NA
CV(%)	NA		NA		NA		NA		NA		NA		NA		NA
Bias(%)	3732.86		3229.80		3183.65		3441.97		3402.71		4091.11		3880.82		3325.07

Expected Concentration	4.0	Bias (%)	30.0	Bias (%)	300.0	Bias (%)
Calculated Concentrations	120.23	2905.77	976.09	3153.63	12160.01	3953.34
	121.77	2944.36	978.62	3162.08	12172.97	3957.66
	120.93	2923.33	992.96	3209.88	12127.73	3942.58

n	3		3		3
Mean	120.98		982.56		12153.57
StdDev	0.77		9.10		23.30
CV(%)	0.64		0.93		0.19
Bias(%)	2924.49		3175.20		3951.19

Sample File Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity L Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity H Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	L/H Ratio Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Exp Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Measured Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
Sample File Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Sequence Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity L Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Intensity H Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	L/H Ratio Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Exp Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Measured Conc Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
20220516_PC5_ClientDigest	2+	GFPSVLR	19.6295	2,206,729.2500	2,356,436.5000	0.94		5609.827
20220516_PC5_neat_R01	2+	GFPSVLR	19.2921	5,915,731.0000	16,710,432.0000	0.35		2129.466
20220516_PC5_neat_R02B	2+	GFPSVLR	19.3968	10,540,858.0000	26,886,846.0000	0.39		2356.713
20220516_PC5_neat_R03	2+	GFPSVLR	19.5404	6,510,984.0000	13,961,708.0000	0.47		2800.685
20220516_Seer_NP01_R01	2+	GFPSVLR	19.4109	581,446.9375	5,123,480.0000	0.11		692.233
20220516_Seer_NP01_R02	2+	GFPSVLR	19.4109	706,169.5000	5,007,754.5000	0.14		856.725
20220516_Seer_NP01_R03	2+	GFPSVLR	19.4010	731,748.0000	5,070,013.5000	0.14		876.524
20220516_Seer_NP01_R04	2+	GFPSVLR	19.4010	756,089.5625	4,918,662.5000	0.15		932.631
20220516_Seer_NP01_R05	2+	GFPSVLR	19.4010	581,966.3125	3,110,499.0000	0.19		1132.082
20220516_Seer_NP02_R01	2+	GFPSVLR	19.4011	7,583,997.5000	4,319,706.5000	1.76		10504.855
20220516_Seer_NP02_R02	2+	GFPSVLR	19.4010	7,163,691.5000	4,094,400.7500	1.75		10468.746
20220516_Seer_NP02_R03	2+	GFPSVLR	19.4010	6,784,013.0000	4,205,910.5000	1.61		9652.157
20220516_Seer_NP02_R04	2+	GFPSVLR	19.4010	7,380,630.0000	4,188,402.7500	1.76		10543.602
20220516_Seer_NP02_R05	2+	GFPSVLR	19.4109	5,776,500.5000	3,719,802.7500	1.55		9293.241
20220516_Seer_NP03_R01	2+	GFPSVLR	19.4010	1,787,384.0000	4,846,707.5000	0.37		2217.715
20220516_Seer_NP03_R02	2+	GFPSVLR	19.4109	1,459,921.8750	4,216,992.5000	0.35		2082.772
20220516_Seer_NP03_R03	2+	GFPSVLR	19.4010	1,624,734.2500	4,312,948.5000	0.38		2265.085
20220516_Seer_NP03_R04	2+	GFPSVLR	19.4010	1,535,567.8750	4,506,509.5000	0.34		2050.174
20220516_Seer_NP03_R05	2+	GFPSVLR	19.4109	1,226,776.5000	3,072,301.5000	0.40		2400.076
20220516_Seer_NP04_R01	2+	GFPSVLR	19.4010	12,809,988.0000	4,529,785.5000	2.83		16912.039
20220516_Seer_NP04_R02	2+	GFPSVLR	19.4208	10,623,682.0000	4,295,246.0000	2.47		14793.258
20220516_Seer_NP04_R03	2+	GFPSVLR	19.4208	11,073,198.0000	4,757,579.0000	2.33		13921.622
20220516_Seer_NP04_R04	2+	GFPSVLR	19.4208	12,353,408.0000	5,595,074.0000	2.21		13207.103
20220516_Seer_NP04_R05	2+	GFPSVLR	19.4505	9,606,434.0000	3,697,379.5000	2.60		15539.073
20220516_Seer_NP05_R01	2+	GFPSVLR	19.4406	2,219,045.5000	5,300,929.0000	0.42		2515.474
20220516_Seer_NP05_R02	2+	GFPSVLR	19.4406	2,743,169.5000	5,755,820.0000	0.48		2861.900
20220516_Seer_NP05_R03	2+	GFPSVLR	19.4612	2,326,777.0000	5,002,924.5000	0.47		2793.141
20220516_Seer_NP05_R04	2+	GFPSVLR	19.4315	2,569,423.0000	5,604,952.5000	0.46		2753.326
20220516_Seer_NP05_R05	2+	GFPSVLR	19.4810	1,477,918.3750	3,227,482.0000	0.46		2750.319
20220518_Seer_QC-A_R01	2+	GFPSVLR	19.6097	473,832.1562	26,680,410.0000	0.02	4.0	120.231
20220518_Seer_QC-A_R02	2+	GFPSVLR	19.6430	446,571.9688	24,784,964.0000	0.02	4.0	121.774
20220518_Seer_QC-A_R03	2+	GFPSVLR	19.6035	433,542.3750	24,251,272.0000	0.02	4.0	120.933
20220518_Seer_QC-B_R01	2+	GFPSVLR	19.6691	3,926,354.5000	24,388,600.0000	0.16	30.0	976.089
20220518_Seer_QC-B_R02	2+	GFPSVLR	19.6331	3,695,659.2500	22,895,334.0000	0.16	30.0	978.623
20220518_Seer_QC-B_R03	2+	GFPSVLR	19.6133	3,749,628.5000	22,889,338.0000	0.16	30.0	992.964
20220518_Seer_QC-C_R01	2+	GFPSVLR	19.6295	50,614,180.0000	24,900,320.0000	2.03	300.0	12160.009
20220518_Seer_QC-C_R02	2+	GFPSVLR	19.6332	48,479,136.0000	23,824,540.0000	2.03	300.0	12172.967
20220518_Seer_QC-C_R03	2+	GFPSVLR	19.6430	48,349,932.0000	23,849,784.0000	2.03	300.0	12127.726
20220518_Seer_Std_A	2+	GFPSVLR	19.6097	107,707.6172	26,575,878.0000	0.00	1.0	38.329
20220518_Seer_Std_B	2+	GFPSVLR	19.5998	306,577.8125	26,498,084.0000	0.01	2.5	83.245
20220518_Seer_Std_C	2+	GFPSVLR	19.6394	617,710.9375	24,595,248.0000	0.03	5.0	164.182
20220518_Seer_Std_D	2+	GFPSVLR	19.6097	2,864,097.7500	24,649,818.0000	0.12	20.0	708.394
20220518_Seer_Std_E	2+	GFPSVLR	19.5899	6,074,950.0000	26,172,026.0000	0.23	40.0	1401.084
20220518_Seer_Std_F	2+	GFPSVLR	19.6394	24,085,212.0000	21,506,942.0000	1.12	160.0	6705.776
20220518_Seer_Std_G	2+	GFPSVLR	19.5899	61,715,268.0000	23,179,680.0000	2.66	400.0	15923.282
20220518_Seer_Std_H	2+	GFPSVLR	19.6035	105,237,056.0000	18,367,078.0000	5.73	1000.0	34250.738

Lower Limit of Detection	NA
Lower Limit of Quantitation	NA
Upper Limit of Quantitation	NA
LOQ Bias Limit	30.0%
LOQ CV Limit	N/A
LOD Calculation	None
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Calibration Curve: GFPSVLR

Calibration Curve

Figures of Merit

Concentrations in Standards

Concentrations in Quality Control

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Summary Charts

Peak Areas

Retention Times

Quantitation Ratios