MRM Proteomics - Seer Proteograph

Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and reaction monitoring mass spectrometry with stable isotope-labelled internal standards
Data License: CC BY 4.0 | ProteomeXchange: PXD053704 | doi: https://doi.org/10.6069/2a58-q964
  • Organism: Homo sapiens
  • Instrument: 6495C Triple Quadrupole LC/MS
  • SpikeIn: Yes
  • Keywords: plasma proteomics, LC/MRM-MS, fractionation, absolute quantitation, Seer Proteograph
  • Lab head: Christoph Borchers Submitter: Claudia Gaither
Abstract
When using a mix of 274 light synthetic peptide standards as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards as normalizers for targeted quantitative analysis by LC/MRM-MS, the Seer Proteograph allowed for the enrichment and absolute quantitation of up to an additional 44% of protein targets (median) as well as improved reproducibility compared to a traditional proteomic workflow with no fractionation (median 8.3% vs. 13.1% CV). As the Proteograph technology gains popularity, establishing Proteograph-specific protein reference ranges and comparisons to methods like ELISA and LC/MRM-MS may be explored to enable absolute quantification of plasma proteins based on Proteograph data.
Created on 7/5/24, 3:57 PM
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