Tulane U Biochem Hu Lab - patients from Italy cohort CFP10 results

MRM quantification of circulating CFP-10 in TB patients
Data License: CC BY 4.0 | ProteomeXchange: PXD021150 | doi: https://doi.org/10.6069/nqnf-qc53
  • Organism: Mycobacterium tuberculosis
  • Instrument: TSQ Altis
  • SpikeIn: Yes
  • Keywords: CFP10 plasma TB
  • Lab head: Tony Hu Submitter: Qingbo Shu
Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting pathogen derived antigens which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and imunoprecipitation of targeted peptides and applied this method in clinical samples.
Created on 11/30/21, 9:14 AM
Clustergrammer Heatmap
CFP-10 standard curve_2021-11-09_12-51-58.sky.zip2021-11-30 09:14:0711214101
NHP TB BAL CFP10_MRM_2021-11-09_12-52-18.sky.zip2021-11-30 09:14:0711214131
CFP-10 LOD estimation_2021-11-09_12-51-33.sky.zip2021-11-30 09:14:0711214101
18 adult TB patient with two sampling points CFP10_2021-11-09_12-49-48.sky.zip2021-11-30 09:14:0711214361
31 pediatric participants from Dominican Republic cohort_2021-11-09_12-50-10.sky.zip2021-11-30 09:14:0711214311
PUSH pediatric cohort CFP10_MRM_2021-11-10_16-33-36.sky.zip2021-11-30 09:14:0711214231
10 adult TB patient with repeating CFP10_2021-11-09_12-49-05.sky.zip2021-11-30 09:14:0711214201
137 adult TB patient CFP10_2021-11-10_14-11-19.sky.zip2021-11-30 09:14:07112141371
NHP TB plasma CFP10_MRM_2021-11-09_12-52-46.sky.zip2021-11-30 09:14:0711214131
CFP-10pep peak feature analysis in spiked-in samples_2021-11-10_10-57-06.sky.zip2021-11-30 09:14:0611214211