Skyline documents |
2024-04-30 |
All Skyline documents used for analysis are included in the Targeted MS Runs section and described briefly below:
| Skyline document | Description | Raw data folder |
| qLITvsqOT_precision_final_2020-12-30_07-35-14.sky.zip | Replicate injections in linear ion trap and Orbitrap using precursor isolation windows of 0.7 and 1.7 Th | replicate_injections |
| qLITvsqOT_matrix_matched_originaltransitions_LIT_2021-04-27_14-24-59.sky.zip | Matrix-matched dilution curves in linear ion trap using original transition set | matrix_matched |
| qLITvsqOT_matrix_matched_originaltransitions_OT_2021-04-27_14-23-49.sky.zip | Matrix-matched dilution curves in Orbitrap using original transition set | matrix_matched |
| qLITvsqOT_matrix_matched_alltransitions_LIT_2021-04-27_14-13-12.sky.zip | Matrix-matched dilution curves in linear ion trap with all possible transitions | matrix_matched |
| qLITvsqOT_matrix_matched_alltransitions_OT_2021-04-27_14-18-08.sky.zip | Matrix-matched dilution curves in Orbitrap with all possible transitions | matrix_matched |
Subsequent processing of the data was done externally, and with the code available here. Skyline documents include calibration curves, but limit of quantitation was determined externally with the method described by Pino et al. (2020).