Whole blood peptide assay stability by MRM-MS
Gaither C, Popp R, Zahedi RP, Borchers CH. Multiple Reaction Monitoring-Mass Spectrometry Enables Robust Quantitation of Plasma Proteins Regardless of Whole Blood Processing Delays That May Occur in the Clinic. Mol Cell Proteomics. 2022 May;21(5):100212. doi: 10.1016/j.mcpro.2022.100212. Epub 2022 Feb 17. PMID: 35182769; PMCID: PMC9062485.
- Organism: Homo sapiens
- Instrument: 6495B Triple Quadrupole LC/MS
- SpikeIn:
Yes
- Keywords:
whole blood, mass spectrometry, MRM, plasma, proteomics, internal standards
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Lab head: Christoph Borchers
Submitter: Claudia Gaither
Here, we investigate how delays in plasma processing affect peptide-centric “bottom-up” proteomics, using multiple reaction monitoring mass spectrometry (MRM-MS). We used validated Tier 2-level assays for proteotypic peptide surrogates of 270 human proteins to analyze plasma generated from whole blood that has been kept at room temperature from 0-40 hours before plasma generation. Moreover, we evaluated whether different plasma thawing conditions had an impact on the MRM-based plasma protein measurements.
Created on 8/16/21, 6:12 PM