2020_Human BAK125 kit _Room Temperature
Gaither C, Popp R, Borchers SP, Skarphedinsson K, Eiriksson FF, Thorsteinsdóttir M, Mohammed Y, Borchers CH. Performance Assessment of a 125 Human Plasma Peptide Mixture Stored at Room Temperature for Multiple Reaction Monitoring-Mass Spectrometry. J Proteome Res. 2021 Sep 3;20(9):4292-4302. doi: 10.1021/acs.jproteome.1c00249. Epub 2021 Jul 16. PMID: 34270269.
- Organism: Homo sapiens
- Instrument: 6495B Triple Quadrupole LC/MS,Xevo TQ-XS
- SpikeIn:
Yes
- Keywords:
multiple reaction monitoring, MRM, mass spectrometry, peptide stability, proteomics, targeted proteomics, internal standards
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Lab head: Christoph Borchers
Submitter: Claudia Gaither
We assessed the impact of leaving a lyophilized mixture consisting of 125 peptides at room temperature for up to 20 days, and we assessed the effect on the quantitative performance of multiple reaction monitoring-MS (MRM-MS) assays. The findings suggest that there are no significant differences in the MRM-MS results for the time points assessed in this study (up to 20 days).
A lyophilized peptide mixture was transported from Canada to Iceland without dry ice and comparison between analyses at both sites were made.
Created on 8/30/21, 9:06 AM