Validation of proteins showing significant dysregulation from shotgun proteomics experiments in longitudinal plasma samples of COVID-19 patients
Suvarna K, Salkar A, Palanivel V, Bankar R, Banerjee N, Gayathri J Pai M, Srivastava A, Singh A, Khatri H, Agrawal S, Shrivastav O, Shastri J, Srivastava S. A Multi-omics Longitudinal Study Reveals Alteration of the Leukocyte Activation Pathway in COVID-19 Patients. J Proteome Res. 2021 Oct 1;20(10):4667-4680. doi: 10.1021/acs.jproteome.1c00215. Epub 2021 Aug 11. PMID: 34379420; PMCID: PMC8370121.
- Organism: Homo sapiens
- Instrument: TSQ Altis
- SpikeIn:
Yes
- Keywords:
COVID-19, SARS-CoV-2, mass-spectrometry, longitudinal, proteomics, metabolomics
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Lab head: Sanjeeva Srivastava
Submitter: Sanjeeva Srivastava
A Label Free Quantification study was done to observe the changes in the plasma proteome of patient samples collected at two different time points of disease severity (T1 and T2). A few of the proteins that were significantly dysregulated between the two time points were taken forward for validation using the Multiple Reaction Monitoring approach. The Skyline file here shows two proteins: 10 Vitamin D binding protein and Profilin 1.
This data is part of a paper titled "A multi-omics longitudinal study reveals alteration of leukocyte activation pathway in COVID-19 patients".
Created on 6/21/21, 7:50 PM