IIT Bombay Proteomics - COVID-19_Semen_Proteomics_Host

Targeted validation of significantly altered semen proteins in COVID-19 patients
Data License: CC BY 4.0 | ProteomeXchange: PXD026849 | doi: https://doi.org/10.6069/shzj-bp03
  • Organism: Homo sapiens
  • Instrument: TSQ Altis
  • SpikeIn: Yes
  • Keywords: SARS-CoV-2, COVID-19, SRM/MRM, Proteomics, Semen
  • Lab head: Sanjeeva Srivastava Submitter: Sanjeeva Srivastava
Abstract
The current global outbreak of COVID-19 has significantly impacted several organ systems. Male reproductive organs are among the potential targets of SARS-CoV-2 infection owing to the presence of abundant viral receptors; ACE2 and TMPRSS2 in the testis. However, the questions pertaining to the long term effects of SARS-CoV-2 infection on male fertility are still unanswered. For this study, we procured the semen samples from COVID-19 recovered patients and performed mass spectrometry based proteomic studies to understand the impact of the disease on the biological processes involved in reproductive health.
Experiment Description
The downregulated proteins in COVID-19 recovered patients obtained from the shotgun proteomics analysis were considered for targeted validation using SRM/MRM based approach. Transition list was prepared using Skyline software and data was acquired in Triple Quadrupole Altis (Thermo Scientific) coupled with uHPLC system.
Sample Description
Samples were collected from 27 individuals (10 healthy fertile controls and 17 COVID-19 recovered males).
Created on 6/21/21, 7:57 PM
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Semen_Host_DR_MRM_individual_samples_2021-06-18_18-31-08.sky.zip2021-06-21 19:56:519545439932
Semen_Host_DR_MRM_pool_2021-06-18_18-24-19.sky.zip2021-06-21 19:56:51954543995