Targeted validation of significantly altered semen proteins in COVID-19 patients
Ghosh S, Parikh S, Nissa MU, Acharjee A, Singh A, Patwa D, Makwana P, Athalye A, Barpanda A, Laloraya M, Srivastava S, Parikh F. Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function. ACS Omega. 2022 Mar 7;7(10):8601-8612. doi: 10.1021/acsomega.1c06551. PMID: 35309488; PMCID: PMC8928495.
- Organism: Homo sapiens
- Instrument: TSQ Altis
- SpikeIn:
Yes
- Keywords:
SARS-CoV-2, COVID-19, SRM/MRM, Proteomics, Semen
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Lab head: Sanjeeva Srivastava
Submitter: Sanjeeva Srivastava
The current global outbreak of COVID-19 has significantly impacted several organ systems. Male reproductive organs are among the potential targets of SARS-CoV-2 infection owing to the presence of abundant viral receptors; ACE2 and TMPRSS2 in the testis. However, the questions pertaining to the long term effects of SARS-CoV-2 infection on male fertility are still unanswered.
For this study, we procured the semen samples from COVID-19 recovered patients and performed mass spectrometry based proteomic studies to understand the impact of the disease on the biological processes involved in reproductive health.
The downregulated proteins in COVID-19 recovered patients obtained from the shotgun proteomics analysis were considered for targeted validation using SRM/MRM based approach. Transition list was prepared using Skyline software and data was acquired in Triple Quadrupole Altis (Thermo Scientific) coupled with uHPLC system.
Samples were collected from 27 individuals (10 healthy fertile controls and 17 COVID-19 recovered males).
Created on 6/21/21, 7:57 PM