Standardized Workflow for Proteomic Analysis of blood biofluids.

(A) Overview of steps in the standardized sample preparation protocol. This protocol was assessed using naive plasma (green), depleted plasma (blue) and dried blood spots (red). Each sample type was evaluated in a high-throughput (21-min gradient, 25 mins/sample) and mid-throughput (60-min gradient, 72 mins/sample) DIA-MS analysis.