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FLNc_S2233_mutants_Phosphopeptides_2018-02-28_15-24-07.sky.zip

Warscheid Lab - Targeted FLNc S2233 S2236 phosphorylation assay

Document Summary

 
 
9 proteins, 12 peptides, 13 precursors, 78 transitions  -  12 replicates  -  Skyline (64-bit) 4.1.0.11796

Transition List

 
 
sp|Q9UKE5|TNIK_HUMAN TRAF2 and NCK-interacting protein kinase OS=Homo sapiens GN=TNIK PE=1 SV=1
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+M714.3320 
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+M+1714.8334 
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+M+2715.3347 
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+y7791.44101+
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+y6678.35691+
SEGSPVLPHEPAK01426.6494 SEGSPVLPHEPAKlight1426.6494714.33202+y3315.20271+
sp|Q86YS7|C2CD5_HUMAN C2 domain-containing protein 5 OS=Homo sapiens GN=C2CD5 PE=1 SV=1
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+M516.5568 
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+M+1516.8911 
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+M+2517.2250 
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+y8819.43591+
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+y5505.27691+
TGMGSGSAGKEGGPFK11546.6487 TGMGSGSAGKEGGPFKlight1546.6487516.55683+y1147.11281+
hFLNc WT  
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+M651.8137 
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+M+1652.3151 
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+M+2652.8164 
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+M602.8253 
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+y6680.37261+
ERLGSFGSITR11301.6129 ERLGSFGSITRlight1301.6129651.81372+y1175.11901+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M509.2419 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M+1509.7433 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M+2510.2446 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y8806.41551+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y5533.30421+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y4476.28271+
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+M549.2250 
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+M+1549.7265 
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+M+2550.2277 
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+y5515.29361+
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+y2276.16661+
LGSFGSITR01096.4355 LGSFGSITRlight1096.4355549.22502+y1175.11901+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M509.2419 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M+1509.7433 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+M+2510.2446 
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y8806.41551+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y5515.29361+
LGSFGSITR01016.4692 LGSFGSITRlight1016.4692509.24192+y1175.11901+
hFLNc S2233A  
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+M501.2444 
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+M+1501.7459 
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+M+2502.2471 
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+y8790.42061+
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+y6662.36201+
LGAFGSITR01000.4743 LGAFGSITRlight1000.4743501.24442+y5515.29361+
hFLNc S2233D  
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+M523.2393 
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+M+1523.7408 
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+M+2524.2421 
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+y8834.41041+
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+y6662.36201+
LGDFGSITR01044.4641 LGDFGSITRlight1044.4641523.23932+y5515.29361+
sp|P08631|HCK_HUMAN Tyrosine-protein kinase HCK OS=Homo sapiens GN=HCK PE=1 SV=5
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+M645.2741 
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+M+1645.7756 
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+M+2646.2769 
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+y81077.38851+
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+y7948.34591+
VIEDNEYTAR01288.5337 VIEDNEYTARlight1288.5337645.27412+y1175.11901+
sp|P06241|FYN_HUMAN Tyrosine-protein kinase Fyn OS=Homo sapiens GN=FYN PE=1 SV=3
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+M652.2819 
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+M+1652.7834 
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+M+2653.2847 
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+y81077.38851+
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+y7948.34591+
LIEDNEYTAR01302.5493 LIEDNEYTARlight1302.5493652.28192+y6833.31891+
sp|P50613|CDK7_HUMAN Cyclin-dependent kinase 7 OS=Homo sapiens GN=CDK7 PE=1 SV=1
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+M577.7713 
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+M+1578.2728 
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+M+2578.7740 
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+M528.7829 
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+y5602.36201+
AYTHQVVTR01153.5281 AYTHQVVTRlight1153.5281577.77132+y4474.30351+
sp|P16083|NQO2_HUMAN Ribosyldihydronicotinamide dehydrogenase [quinone] OS=Homo sapiens GN=NQO2 PE=1 SV=5
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+M908.4355 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+M+1908.9369 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+M+2909.4382 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+M859.4470 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+y91002.52141+
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564908.43552+y7789.41011+
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+M605.9594 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+M+1606.2937 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+M+2606.6279 
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+y7789.41011+
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+y6718.37301+
SFNGSLKNVAVDELSR11814.8564 SFNGSLKNVAVDELSRlight1814.8564605.95943+y5619.30461+