Leibniz Institute - Isopeptides of gluten protein types

Identification of Isopeptides Between Human Tissue Transglutaminase and Wheat, Rye, and Barley Gluten Peptides

  • Organism: common wheat, rye, barley, Homo sapiens
  • Instrument: Q Exactive HF
  • SpikeIn: No
  • Keywords: celiac disease, gluten, isopeptides, mass spectrometry, transglutaminase
  • Lab head: Katharina Scherf
Celiac disease (CD) is a chronic immune-mediated enteropathy of the small intestine, which is triggered by the ingestion of storage proteins (gluten) from wheat, rye, and barley in genetically predisposed individuals. Human tissue transglutaminase (TG2) plays a central role in the pathogenesis of CD, because it is responsible for specific gluten peptide deamidation and covalent crosslinking between glutamine and lysine, resulting in the formation of Nε-(γ-glutamyl)-lysine isopeptide bonds. The resulting TG2-gluten peptide complexes are assumed to cause the secretion of anti-TG2-autoantibodies, but the underlying mechanisms are only partly known. To gain more insight into the structures of these complexes, the aim of our study was to identify TG2-gluten isopeptides. We applied a reciprocal proteomics strategy including discovery-driven as well as targeted proteomics to complex wheat, rye and barley gluten hydrolysates that had been incubated with TG2. In total, we detected 29 TG2-gluten isopeptides, involving seven selected TG2 lysine residues (K205, K265, K429, K468, K590, K600, K677). Several gluten peptides carried known B-cell epitopes and/or T-cell epitopes, either intact 9-mer core regions or partial sequences, as well as sequences bearing striking similarities to already known epitopes. These novel insights into the molecular structures of TG2-gluten peptide complexes may help clarify their physiological relevance in the initiation of CD autoimmunity and the role of anti-TG2 autoantibodies
Created on 1/28/20, 10:26 AM