Bankar R, Suvarna K, Ghantasala S, Banerjee A, Biswas D, Choudhury M, Palanivel V, Salkar A, Verma A, Singh A, Mukherjee A, Pai MGJ, Roy J, Srivastava A, Badaya A, Agrawal S, Shrivastav O, Shastri J, Srivastava S. Proteomic investigation reveals dominant alterations of neutrophil degranulation and mRNA translation pathways in patients with COVID-19. iScience. 2021 Mar 19;24(3):102135. doi: 10.1016/j.isci.2021.102135. Epub 2021 Feb 4. PMID: 33558857; PMCID: PMC7857979.
In the current scenario of COVID-19, an assay that can efficiently and specifically detect infection by SARS-COV2 is required. Nasopharyngeal swabs are non-invasive samples and can be conveniently acquired from the population to be screened. In this work, we have validated a few viral and host peptides (found to be relevant from our shotgun data) using a Triple Quadrupole mass spectrometer.
A subset of the raw data files and search results used to build the Bibliospec spectrum library used in this analysis are available in the PRIDE repository with the accession PXD020580.