We created a hypermethylated DIA assay library by DDA acquisitions of 6 SCX fractions from Gnmt -/- mouse livers.  We supplemented this library with a DDA acquisition of a methyl-Lys immunoprecipitation from a hepatoma cell line derived from Gnmt -/- mice. This gave us an assay library of 808 methyl Arg and Lys peptides. We further supplemented this library with a DDA acquisition of a ‘one-pot’ acetyl-Lys and succinyl-Lys immunoaffinity enrichment of liver mitochondrial extract and whole liver lysate which provided us with 2428­­ succinylated peptides and 2547 acetylated peptides.     The Skyline and OpenSWATH assay libraries normalized to ‘indexed’ retention time space are avaliable here for further studies.