Duke - p400HR Ring Trial System Suitability

Duke - p400HR Ring Trial System Suitability
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Streptavidin Peptides_2020-09-30_15-13-28.sky.zip2020-10-27 13:35:321082400
BSA-QC_68fmol_Sep2020_Impact-II_2020-10-03_14-17-45.sky.zip2020-10-03 14:18:51104021660
Stergachis-SupplementaryData_2_b.sky2020-09-30 16:33:3110012487610
Stergachis-SupplementaryData_2_a.sky2020-09-30 16:32:3510011584610
Genome-scale metabolic rewiring to improve titers, rates and yields of the non-native non-ribosomal peptide product indigoidine at scale_2020-02-17_12-40-25.sky.zip2020-09-18 14:56:4716041186450
iPSC_Markers-LINCS-v4-2020august12_2020-08-14_19-21-16.sky.zip2020-09-15 09:50:3418606,03437,44460
Tier3_2020-09-10_18-48-08.sky.zip2020-09-13 11:03:1120398693
200720_0845_2020-09-10_18-55-02.sky.zip2020-09-13 11:02:0720515453
200721_1119_2020-09-10_18-56-20.sky.zip2020-09-13 11:02:0720515453
200722_1408_2020-09-10_18-57-32.sky.zip2020-09-13 11:02:0720515373
200723_1126_2020-09-10_18-58-38.sky.zip2020-09-13 11:02:0720515443
200724_1047_2020-09-10_18-59-46.sky.zip2020-09-13 11:02:0720515413
200727_1254r_2020-09-10_19-00-53.sky.zip2020-09-13 11:02:0720515443
200728_0950r_2020-09-10_19-09-04.sky.zip2020-09-13 11:02:0720515453
200729_1036_2020-09-10_19-17-39.sky.zip2020-09-13 11:02:0720515463
200804_0953_2020-09-10_19-18-30.sky.zip2020-09-13 11:02:0620515463
200805_1136_2020-09-10_19-19-12.sky.zip2020-09-13 11:02:0620515463
200806_1428_2020-09-10_19-20-01.sky.zip2020-09-13 11:02:0620515353
200820_1557_2020-09-10_19-21-02.sky.zip2020-09-13 11:02:0620515483
200821_1134_2020-09-10_19-21-42.sky.zip2020-09-13 11:02:0620515393
200821_1358_2020-09-10_19-22-30.sky.zip2020-09-13 11:02:0620515363
PRM_9min_2020-09-11_15-50-27.sky.zip2020-09-13 11:01:282096689
PRM_60min_2020-09-11_15-51-41.sky.zip2020-09-13 11:01:284020122817
OSCA_TiO2enrich_12_OrderedBySample_Panorama_2020-08-08_17-37-31.sky.zip2020-09-10 07:07:581002191270
BSA-QC_68fmol_Aug2020_Impact-II_2020-09-02_08-08-32.sky.zip2020-09-02 08:10:11104021650
LMR0015_Oreni-Kidney_minPanorama_2020-08-28_19-19-59.sky.zip2020-08-31 23:56:542,12009,22652,361120
microglia_grouped_2020-07-07_10-11-36.sky.zip2020-08-30 20:23:302,918033,602100,806150
BP_MyoMuscle_241017_2020-06-01_21-42-31.sky.zip2020-08-17 15:30:326110110110160
Determine_linkage_BP_2020-06-01_23-44-28.sky.zip2020-08-17 15:30:32266630
BP_CA_Nglycanlibrary_final_2020-06-03_01-02-34.sky.zip2020-08-17 15:30:324898989240
BP_GlycoT_Quantcheck_addstruc_2020-06-03_01-13-03.sky.zip2020-08-17 15:30:324979797120
E4HN 69 Wk F_M_120k.sky.zip2020-08-11 12:22:462142839840
E4HN 69 Wk F_M_240k_for Cit+9.sky.zip2020-08-11 12:22:391366840
Pilot Experiment APOE3 3 Mice (21 DBS_3Plasma_2Brain).sky.zip2020-08-11 12:22:362142738290
Pilot Experiment APOE3 3 Mice (21 DBS_3Plasma_2Brain)_240k.sky.zip2020-08-11 12:22:331366250
Pilot Kinetics Experiment CVN Mice 120k.sky.zip2020-08-11 12:22:32210182480
5572_Plate1LC2_07232020.sky.zip2020-08-11 12:19:389505050990
5572_Plate2LC1_07222020.sky.zip2020-08-11 12:19:2317512212311375
5572_Plate2LC2_07232020.sky.zip2020-08-11 12:18:499505050990
5572_Plate3LC1_07282020.sky.zip2020-08-11 12:18:3217512212311375
5572_Plate3LC2_07282020.sky.zip2020-08-11 12:17:559505050990
5572_Plate4LC1_07302020.sky.zip2020-08-11 12:17:3517512212311375
5572_Plate4LC2_07312020.sky.zip2020-08-11 12:17:009505050990
5572_Plate1LC1_07222020.sky.zip2020-08-11 12:16:4517512212311375
vanGelder_Kinase_phosphorylation_2020-07-30_16-35-41.sky.zip2020-08-06 12:03:1440105020
Common_Wheat_2020-07-30_13-16-10.sky.zip2020-08-03 08:35:4013044186480
Representative_Response_2020-07-30_13-25-50.sky.zip2020-08-03 08:35:401304321370
Durum_Wheat_2020-07-30_13-31-28.sky.zip2020-08-03 08:35:4013044196480
Einkorn_2020-07-30_13-53-08.sky.zip2020-08-03 08:35:4013044190540
Emmer_2020-07-30_13-58-02.sky.zip2020-08-03 08:35:4013044188510
Spelt_2020-07-30_14-09-32.sky.zip2020-08-03 08:35:4013044182480
Identification_Peptides_2020-07-21_14-05-35.sky.zip2020-08-03 08:32:1713064839160
Identification_Peptides_Selection_2020-07-30_14-12-39.sky.zip2020-08-03 08:32:171203517540
BSA-QC_68fmol_Jul2020_Impact-II_minPanorama_2020-07-30_12-55-38.sky.zip2020-07-30 12:57:29104021670
NIST PTM.sky.zip2020-07-29 09:37:022013741130
200626_PRM_Validation_Samples_FilteredAssays_2020-07-13_11-22-54.sky.zip2020-07-22 15:25:2568037694170
Twiss_PRM_unmod_24.sky.zip2020-07-20 05:19:408401841,713330
LINCS_P100_DIA_Plate97_annotated_minimized_2020-06-29_18-13-03.sky.zip2020-06-29 15:20:449001922,7649096
BSA-QC_68fmol_Jun2020_Impact-II_minPanorama_2020-06-28_22-05-23.sky.zip2020-06-28 22:10:23104021650
19_8_27_LARP1_Kinase_Reaction_2020-06-18_10-51-35.sky.zip2020-06-24 21:25:59207623730
Phospho_CDK2_Silencing_Experiment_Human_2020-06-23_16-21-00.sky.zip2020-06-24 21:25:5910216360
20_6_Phospho_SAMe_Combined.sky.zip2020-06-24 21:25:582,779030,00390,009330
Sky0 ACE2 TMPRSS2 cell lines_2020-06-18_16-55-27.sky.zip2020-06-18 13:38:456029197320
LINCS_P100_DIA_Plate96_annotated_minimized_2020-06-11_19-02-30.sky.zip2020-06-11 16:24:329001922,7649396
AMBPb_2020-06-01_14-39-27.sky.zip2020-06-11 15:34:13102610
AMBP_2020-06-01_17-29-42.sky.zip2020-06-11 15:34:131026680
AMBPc_2020-06-02_12-38-26.sky.zip2020-06-11 15:34:13102620
B2Mb_2020-06-02_15-19-17.sky.zip2020-06-11 15:34:13102680
B2M_2020-06-02_17-56-29.sky.zip2020-06-11 15:34:131026630
HBB_2020-06-03_11-25-07.sky.zip2020-06-11 15:34:131026720
IGHA1_2020-06-03_14-07-57.sky.zip2020-06-11 15:34:1310210690
IGHA1b_2020-06-03_16-47-13.sky.zip2020-06-11 15:34:131021010
IGHA1c_2020-06-04_12-45-14.sky.zip2020-06-11 15:34:131021020
LYZ_2020-06-04_15-26-23.sky.zip2020-06-11 15:34:131024600
LYZb_2020-06-04_18-12-30.sky.zip2020-06-11 15:34:131024120
SERPINF1_2020-06-05_12-55-50.sky.zip2020-06-11 15:34:131026690
SERPINF1b_2020-06-05_16-03-56.sky.zip2020-06-11 15:34:13102620
Sky1 Synthetic SARS-CoV2 proteome library_2020-05-30_00-33-22.sky.zip2020-06-09 13:21:561201351,21210
Sky3 52 patients nano PRM-MS2 raw_2020-05-30_00-38-21.sky.zip2020-06-09 13:20:236057340550
Sky4 5 positive patients nano PRM-MS2 refined_2020-05-30_00-52-02.sky.zip2020-06-09 13:20:22604929450
Sky6 11 positive patients micro PRM-MS2 refined_2020-05-30_00-19-04.sky.zip2020-06-09 13:11:206055273110
Sky5 91 patients micro PRM-MS2 raw_2020-05-30_00-57-41.sky.zip2020-06-09 13:11:206055273910
Sky2 Dilution series SARS Cov2 nano and microflow_2020-05-30_00-30-29.sky.zip2020-06-09 13:04:536057371180
20191031_Cdc55AID_PRM_l1_manual3_2020-05-02_10-59-22.sky.zip2020-06-02 20:16:492805621640
20191031_Cdc55AID_PRM_l2_manual3_2020-05-13_10-32-35.sky.zip2020-06-02 20:16:492605420640
20191031_Cdc55AID_PRM_l3_manual3_2020-05-13_11-25-38.sky.zip2020-06-02 20:16:492404818240
STORI_COHORT_2020-06-02_14-14-43.sky.zip2020-06-02 05:40:567301546161020
Intra-day QC_2020-06-02_14-13-40.sky.zip2020-06-02 05:39:4573015461660
Inter-day QC_2020-06-02_14-12-41.sky.zip2020-06-02 05:39:3473015461650
Instrumental set-up QC_2020-06-02_14-10-53.sky.zip2020-06-02 05:39:2373015461650
ARMeD_NEDP1_2019-11-02_13-41-15.sky.zip2020-05-25 20:17:537501622,589180
LINCS_P100_DIA_Plate29_annotated_minimized_2020-05-18_18-50-11.sky.zip2020-05-18 15:59:169001921,0289696
DIAassayLibrary_Gasac-Gill_Rev2_2020-05-15_18-31-25.sky.zip2020-05-15 19:31:421,69104,87926,12300
DIAassayLibrary_Gasac-Gill_Rev1_2020-05-15_17-01-05.sky.zip2020-05-15 17:38:291,50705,10425,32200
Inflammatory_peptides_2020-05-13_13-35-09.sky.zip2020-05-13 10:58:1317049218600
Manually_filtered_peptides_2020-05-13_08-27-56.sky.zip2020-05-13 05:51:5862503,20513,126600
Prefiltering_CSF_document_2020-05-12_09-28-28.sky.zip2020-05-12 07:21:051,22205,95422,911660
ResponseCurve-EGFR-LTQLGTFEDHFLSLQR_2020-01-28_09-46-19.sky.zip2020-05-11 16:35:091024340
ResponseCurve-CCND1-AYPDANLLNDR_2020-01-28_09-46-04.sky.zip2020-05-11 16:35:091026440
ResponseCurve-FOLH1-TYSVSFDSLFSAVK_2020-01-28_09-46-45.sky.zip2020-05-11 16:35:091026380
International Ring Trial of a Broad-Spectrum Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis [System Suitability Files]

  • Organism: Homo sapiens
  • Instrument: Q Exactive HF,Q Exactive Plus,Q Exactive
  • SpikeIn: Yes
  • Keywords: p400HR, targeted, metabolomics, Q Exactive, Biocrates
  • Lab head: Will Thompson
Abstract
A significant challenge facing the vision of metabolomics in precision medicine, and the analysis of large human cohorts, is the cross-laboratory and longitudinal comparability of quantitative measurements and laboratory protocols used. In this study, 14 laboratories world-wide analyzed various blood specimens using a common experimental protocol provided with Biocrates AbsoluteIDQ p400HR kit to quantify up to 408 metabolites. The specimens included human plasma and serum from male and female donors, mouse and rat plasma as well as NIST SRM 1950 reference plasma. The metabolite targets covered from very polar (amino acids and biogenic amines), to very nonpolar (diacyl- and triacyl-glycerols), and spanned across 11 common metabolite classes. The manuscript describes a strict system suitability testing (SST) criteria used to first evaluate each laboratory’s readiness to perform the assay, and provides the SST Skyline documents for public dissemination. Approximately 250 metabolites were routinely quantified with the kit using various models of high-resolution Orbitrap instruments. Inter-laboratory variance for the NIST SRM-1950 has a median of 10% for amino acids, 24% for biogenic amines, 38% for acylcarnitines, 25% for glycerolipids, 23% for glycerophospholipids, 16% for cholesteryl esters, 15% for sphingolipids, and 9% for hexoses. This study provides recommendations for large metabolomics studies in terms of best practices for system suitability, quality control, and calibration; and has demonstrated that high-resolution metabolomics methods provide excellent quantitative precision across laboratories.
Experiment Description
This documentation includes two Skyline files, one for the UHPLC-MS measurement of amino acids and biogenic amines using the kits, and a second using flow-injeciton analysis (FIA)-MS for the purposes of testing measurement capability for acylcarnitines, hexoses, and lipids. The SST solution is made up of a mix of stable-isotope labeled standards.
Sample Description
A mix of neat standards distributed with each kit for the purposes of establishing System Suitability prior to attempting the p400HR analysis.
Created on 4/12/19, 3:54 PM