Duke - p400HR Ring Trial System Suitability

Duke - p400HR Ring Trial System Suitability
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TPAD_CSF1A_Batch1-multiNF-DIANN.sky.zip2025-07-01 17:49:472,85327,128032,575259,532430
TPAD_CSF1A_Batch1-multiNF-DIANN-grouped.sky.zip2025-07-01 17:41:312,67226,439031,769253,119430
TPAD_CSF1B_Batch2-multiNF-DIANN.sky.zip2025-07-01 15:26:132,85327,128032,575259,532410
TPAD_CSF1B_Batch2-multiNF-DIANN-grouped.sky.zip2025-07-01 15:17:422,67226,439031,769253,119410
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TPAD_CSF2B_Batch4-multiNF-DIANN.sky.zip2025-07-01 11:21:372,85327,128032,575259,532430
TPAD_CSF2B_Batch4-multiNF-DIANN-grouped.sky.zip2025-07-01 11:09:482,67226,439031,769253,119430
TPAD_CSF3A_Batch5-multiNF-DIANN.sky.zip2025-06-30 15:31:332,85327,128032,575259,532430
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TPAD_CSF3B_Batch6-multiNF-DIANN.sky.zip2025-06-30 14:43:542,85327,128032,575259,532410
TPAD_CSF3B_Batch6-multiNF-DIANN-grouped.sky.zip2025-06-30 14:36:142,67226,439031,769253,119410
TPAD_CSF4_Batch7-multiNF-DIANN.sky.zip2025-06-30 13:55:172,85327,128032,575259,532420
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TPAD_CSF5_Batch8-multiNF-DIANN.sky.zip2025-06-30 13:02:062,85327,128032,575259,532540
TPAD_CSF5_Batch8-multiNF-DIANN-grouped.sky.zip2025-06-30 11:52:172,67226,439031,769253,119540
METRIC_Eclipse_PSB_All_QCs_2025-06-27_01-27-04.sky.zip2025-06-26 22:27:15160662336
Exp_CSF_GPF_A_2024-05-06_21-16-47.sky.zip2025-06-25 18:12:201,8297,37807,37855,04010
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CSF_neurod105_assay_individuals_manual_2024-06-03_15-26-36.sky.zip2025-06-25 18:12:2010290209026,345370
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Aducanumab_Selectivity_2025-06-19_12-58-48.sky.zip2025-06-19 09:59:064408512364
hela_isolation_window_concentration_2000ng.sky.zip2025-06-02 18:36:1910,365188,9470223,7653,101,148180
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hela_isolation_window_experiment_500ng.sky.zip2025-06-02 18:36:1910,364188,9470223,7653,101,148180
hela_isolation_window_experiment_1000ng.sky.zip2025-06-02 18:36:1910,365188,9470223,7653,101,148180
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hela_isolation_window_concentration_2000ng.sky.zip2025-05-30 15:25:4610,365188,9470223,7653,101,148180
ecoli_large_replicates_loaded_refined_2024-05-30_14-02-04.sky.zip2025-05-30 12:00:1836698909894,94080
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ecoli_large_replicates_2024-05-30_14-00-05.sky.zip2025-05-30 12:00:184611,30201,3027,52400
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pq500_100spd_plasma_multireplicate_results_2024-05-30_13-45-07.sky.zip2025-05-30 11:58:4157981808187,65120
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pq500_100spd_neat_multireplicate_results_refined_2024-05-30_13-43-42.sky.zip2025-05-30 11:58:4157981808187,68910
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pq500_100spd_neat_multireplicate_results_2024-05-30_13-42-26.sky.zip2025-05-30 11:58:41579818081811,08810
pq500_60spd_neat_multireplicate_results_2024-05-30_13-41-26.sky.zip2025-05-30 11:58:41579818081811,08810
pq500_60spd_neat_multireplicate_2024-05-30_13-40-53.sky.zip2025-05-30 11:58:41579818081811,08800
hela_isolation_window_experiment_200ng.sky.zip2025-05-30 01:29:3910,365188,9470223,7653,101,148180
hela_isolation_window_experiment_500ng.sky.zip2025-05-30 00:53:5310,364188,9470223,7653,101,148180
hela_isolation_window_experiment_1000ng.sky.zip2025-05-29 23:15:1110,365188,9470223,7653,101,148180
2025-0405-MMCC-EV-Astral-noimpute_2025-05-29_11-52-05.sky.zip2025-05-29 12:13:324,90737,547042,331335,891420
2025-0405-MMCC-EV-Actis-noimpute_2025-05-29_11-46-45.sky.zip2025-05-29 12:03:295,18541,978047,810380,072420
20240709_OBIPhA.sky.zip2025-05-16 09:25:51333037235120
Swaney-IPMS-Data-DIANN_2025-05-15_11-53-43.sky.zip2025-05-15 12:02:265,18545,781052,836422,661150
Vial Comparison Post 01152025 v1 (not daily)_2025-04-29_15-52-29.sky.zip2025-04-30 21:22:2310242424190
Vial Comparison Pre v2_2025-04-29_15-49-25.sky.zip2025-04-30 21:22:2310242424230
ATI_Samples_2023_2025-04-26_13-39-03.sky.zip2025-04-28 10:30:141324048280420
ATI_Response_2023_2025-04-26_13-25-06.sky.zip2025-04-28 10:30:141324048280180
ATI_Samples_2022_2025-02-20_23-31-07.sky.zip2025-04-28 10:30:101223046268480
ATI_Samples_2021_2025-02-20_23-21-52.sky.zip2025-04-28 10:30:081223046268300
2023_ATIsamples_circe_2025-02-20_13-24-11.sky.zip2025-04-25 14:58:171322043250960
2022_ATIsamples_circe_2025-02-20_13-17-06.sky.zip2025-04-25 14:58:091222043250970
2021_ATIsamples_circe_2025-02-20_13-06-53.sky.zip2025-04-25 14:58:021222043250940
SICK_glyco_DIA_OrbitrapSCE_library_2025-04-19_01-42-54.sky.zip2025-04-19 17:49:212455,79602,69935,9371000
SICK_glyco_DIA_AstralCE_library_2025-04-19_01-01-56.sky.zip2025-04-19 17:49:212568,90803,80054,7291000
051024_PlasmaPilotwithDose_2025-04-10_16-09-16.sky.zip2025-04-10 13:09:411408483664
050124_CortexMatrixSelectivityTesting_2025-04-10_15-56-24.sky.zip2025-04-10 12:56:48140852604
METRIC_Exploris_PSB_All_QCs_2025-04-10_15-22-54.sky.zip2025-04-10 12:22:0916066296
Histonas_654187687vez_2025-04-10_14-40-28.sky.zip2025-04-10 05:47:540000000
MS3_15N_dilution_2025-04-04_14-57-13.sky.zip2025-04-07 04:48:067710137760510
Transition_1_Astral_Stellar_second_set_cut_off.sky.zip2025-04-07 04:48:069013,11003,57514,817220
Transition_1_Astral_Stellar_second_set.sky.zip2025-04-07 04:48:069014,73105,43722,646220
pdDiscoverer_GPF.sky.zip2025-04-07 04:48:063962,45203,54842,21860
Intermediate_1_Astral_Stellar_second_subset_CV30.sky.zip2025-04-07 04:48:064811,99702,22411,47780
Intermediate_1_Astral_Stellar_second_subset.sky.zip2025-04-07 04:48:065532,50002,82314,40280
GPF_plasma_refined_210prot.sky.zip2025-04-07 04:48:0621068407394,50030
GPF_plasma_intermediate.sky.zip2025-04-07 04:48:063221,57001,90712,75290
GPF_plasma.sky.zip2025-04-07 04:48:063221,57001,90712,75290
final_assay_Astral_Stellar_410.sky.zip2025-04-07 04:48:064091,23601,3056,24430
all15N_peptides_absolute_quantification.sky.zip2025-04-07 04:48:06756011249830
15N_dilution.sky.zip2025-04-07 04:48:067570114654520
Unknown-HMW-GS-Combination_2024-12-12_13-43-29.sky.zip2025-04-01 14:47:311440040392580
Known-HMW-GS-Combination_2024-12-12_13-15-23.sky.zip2025-04-01 14:47:261440040392370
07_Final-Method_Pooled-Sample_2024-12-12_13-01-40.sky.zip2025-04-01 14:47:21144004038140
06_Refinement-Method_Pooled-Sample_2024-12-12_13-01-05.sky.zip2025-04-01 14:47:2114670671,01640
International Ring Trial of a Broad-Spectrum Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis [System Suitability Files]
  • Organism: Homo sapiens
  • Instrument: Q Exactive HF,Q Exactive Plus,Q Exactive
  • SpikeIn: Yes
  • Keywords: p400HR, targeted, metabolomics, Q Exactive, Biocrates
  • Lab head: Will Thompson Submitter: Will Thompson
Abstract
A significant challenge facing the vision of metabolomics in precision medicine, and the analysis of large human cohorts, is the cross-laboratory and longitudinal comparability of quantitative measurements and laboratory protocols used. In this study, 14 laboratories world-wide analyzed various blood specimens using a common experimental protocol provided with Biocrates AbsoluteIDQ p400HR kit to quantify up to 408 metabolites. The specimens included human plasma and serum from male and female donors, mouse and rat plasma as well as NIST SRM 1950 reference plasma. The metabolite targets covered from very polar (amino acids and biogenic amines), to very nonpolar (diacyl- and triacyl-glycerols), and spanned across 11 common metabolite classes. The manuscript describes a strict system suitability testing (SST) criteria used to first evaluate each laboratory’s readiness to perform the assay, and provides the SST Skyline documents for public dissemination. Approximately 250 metabolites were routinely quantified with the kit using various models of high-resolution Orbitrap instruments. Inter-laboratory variance for the NIST SRM-1950 has a median of 10% for amino acids, 24% for biogenic amines, 38% for acylcarnitines, 25% for glycerolipids, 23% for glycerophospholipids, 16% for cholesteryl esters, 15% for sphingolipids, and 9% for hexoses. This study provides recommendations for large metabolomics studies in terms of best practices for system suitability, quality control, and calibration; and has demonstrated that high-resolution metabolomics methods provide excellent quantitative precision across laboratories.
Experiment Description
This documentation includes two Skyline files, one for the UHPLC-MS measurement of amino acids and biogenic amines using the kits, and a second using flow-injeciton analysis (FIA)-MS for the purposes of testing measurement capability for acylcarnitines, hexoses, and lipids. The SST solution is made up of a mix of stable-isotope labeled standards.
Sample Description
A mix of neat standards distributed with each kit for the purposes of establishing System Suitability prior to attempting the p400HR analysis.
Created on 4/12/19, 3:54 PM