We developed LipidCreator, the first software fully supporting targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses of over 60 lipid classes. LipidCreator provides all functionalities to define fragments, manage stable isotope labeling, optimize collision energy and generate in-silico spectral libraries. LipidCreator proved to be an efficient tool for investigating phospholipid derived mediators to dissect lipid-signaling pathways of human platelets during activation.

Experiment descriptions are available from the following MetaboLights submissions: Targeted Mediator Profiling, QTRAP: https://www.ebi.ac.uk/metabolights/MTBLS1381 DIA Validation, QEx-HF: https://www.ebi.ac.uk/metabolights/MTBLS1382 Targeted Phospho- and Glycerolipid Profiling, QEx-HF: https://www.ebi.ac.uk/metabolights/MTBLS1369

Sample descriptions are available from the following MetaboLights submissions: Targeted Mediator Profiling, QTRAP: https://www.ebi.ac.uk/metabolights/MTBLS1381 DIA Validation, QEx-HF: https://www.ebi.ac.uk/metabolights/MTBLS1382 Targeted Phospho- and Glycerolipid Profiling, QEx-HF: https://www.ebi.ac.uk/metabolights/MTBLS1369