MASS SPEC

2024-03-28

Gonad and hemolymph tissues were collected from 18 geoduck (2 sexes, 3 maturation stages, 3 biological replicates each). Data independent acquisition and peptide centric analysis (PECAN) were used to create SRM transition methods for the Thermo Vantage. A quality control mix (375 fmol of Pierce's peptide retention mix + BSA) was added to each sample and 1 ul of sample peptide was injected in 3 ul. Chromatography was run on a 2 cm C18 trap and 27.5 cm C18 analytical column. Gonad samples were analyzed in triplicate and hemolymph were analyzed in duplicate.