Multiplexed, scheduled high resolution sMRM-HR acquisition on full scan MS/MS instruments integrating data dependent and targeted, quantitative mass spectrometric workflows.

All Acquisitions were performed on a TripleTOF 5600 mass spectrometer:

Data sets uploaded to Panorama:

• MRM-HR - Response curve of 6 Protein Mix in complex matrix/C. elegans lysate (3 reproducibility injections, 2 curve replicates), go to response curve in Panorama, download response curve.

• MRM-HR - System Suitability Study using 6 Protein Mix (10 replicate acquisitions, 3x blanks), go to System Suitability Study in Panorama, download System Suitability Study.

• scheduled sMRM-HR - Reproducibility study of proteins from yeast whole cell lysate (3 replicates), go to yeast reproducibility data set in Panorama, download yeast reproducibility data set.

• scheduled sMRM-HR - Differential expression of proteins comparing E. coli wild type and ackA mutant strains (3 repl. each, WT & ackA), go to ackA/WT data set in Panorama, download  ackA/WT data set.