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hela_isolation_window_experiment_1000ng.sky.zip

Prototype Orbitrap Astral manuscript

Document Summary

 

Precursor List

 
Protein Note/Annotations
 
tr|A0A590UJ96|A0A590UJ96_HUMANUncharacterized protein OS=Homo sapiens OX=9606 PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
GQPVWVETGSGGDPGSR
01684.7805 
GQPVWVETGSGGDPGSR
light2+843.39751684.7805150.0000 
QAVGVSETVGIPR
01311.7147 
QAVGVSETVGIPR
light2+656.86461311.7147150.0000 
APVATEVLLAPR
01235.7238 
APVATEVLLAPR
light2+618.86921235.7238150.0000 
sp|A6NIH7|U119B_HUMANProtein unc-119 homolog B OS=Homo sapiens OX=9606 GN=UNC119B PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
AAAAASAAGPGGLVAGK
01338.7256 
AAAAASAAGPGGLVAGK
light2+670.37011338.7256150.0000 
AAAAASAAGPGGLVAGKEEK
11724.9057 
AAAAASAAGPGGLVAGKEEK
light2+863.46011724.9057150.0000 
AAAAASAAGPGGLVAGKEEK
11724.9057 
AAAAASAAGPGGLVAGKEEK
light3+575.97581724.9057150.0000 
VTENYLCKPEDNIYSIDFTR
02476.1580 
VTENYLCKPEDNIYSIDFTR
light3+826.39332476.1580150.0000 
YQFTPAFLR
01141.5920 
YQFTPAFLR
light2+571.80331141.5920110.0000 
TVGATVEFTVGDKPVSNFR
02023.0375 
TVGATVEFTVGDKPVSNFR
light3+675.35312023.0375150.0000 
NFDFDFGFCIPSSR
01707.7351 
NFDFDFGFCIPSSR
light2+854.87481707.7351150.0000 
NTCEHIYEFPQLSEDVIR
02249.0423 
NTCEHIYEFPQLSEDVIR
light3+750.68802249.0423150.0000 
LMIENPYETR
01264.6122 
LMIENPYETR
light2+633.31341264.6122150.0000 
SDSFYFVDNK
01220.5350 
SDSFYFVDNK
light2+611.27481220.5350120.0000 
sp|O00165|HAX1_HUMANHCLS1-associated protein X-1 OS=Homo sapiens OX=9606 GN=HAX1 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
GFFGFPGPR
0980.4868 
GFFGFPGPR
light2+491.2507980.4868130.0000 
FHSPQHPPEEFGFGFSFSPGGGIR
02617.2139 
FHSPQHPPEEFGFGFSFSPGGGIR
light3+873.41192617.2139150.0000 
FHDNFGFDDLVR
01480.6735 
FHDNFGFDDLVR
light3+494.56511480.6735150.0000 
YPDSHQPR
0998.4570 
YPDSHQPR
light2+500.2358998.4570150.0000 
IFGGVLESDAR
01162.5982 
IFGGVLESDAR
light2+582.30641162.5982150.0000 
SESPQPAPDWGSQRPFHR
02077.9718 
SESPQPAPDWGSQRPFHR
light3+693.66462077.971890.0000 
FDDVWPMDPHPR
01510.6663 
FDDVWPMDPHPR
light2+756.34041510.6663120.0000 
FDDVWPMDPHPR
01510.6663 
FDDVWPMDPHPR
light3+504.56271510.6663140.0000 
EDNDLDSQVSQEGLGPVLQPQPK
02492.2031 
EDNDLDSQVSQEGLGPVLQPQPK
light3+831.74162492.2031150.0000 
ITKPDGIVEER
01255.6772 
ITKPDGIVEER
light2+628.84591255.6772140.0000 
ITKPDGIVEER
01255.6772 
ITKPDGIVEER
light3+419.56631255.6772150.0000 
RTVVDSEGR
11017.5203 
RTVVDSEGR
light2+509.76741017.5203120.0000 
TVVDSEGR
0861.4192 
TVVDSEGR
light2+431.7169861.4192100.0000 
TVVDSEGRTETTVTR
11649.8220 
TVVDSEGRTETTVTR
light3+550.94801649.8220110.0000 
TETTVTR
0806.4134 
TETTVTR
light2+404.2140806.413490.0000 
sp|O14810|CPLX1_HUMANComplexin-1 OS=Homo sapiens OX=9606 GN=CPLX1 PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
EAEAQAAMEANSEGSLTRPK
02088.9746 
EAEAQAAMEANSEGSLTRPK
light3+697.33212088.9746150.0000 
YLPGPLQDMLK
01273.6740 
YLPGPLQDMLK
light2+637.84431273.6740130.0000 
YLPGPLQDMLKK
11401.7690 
YLPGPLQDMLKK
light3+468.26361401.7690140.0000 
sp|O15014|ZN609_HUMANZinc finger protein 609 OS=Homo sapiens OX=9606 GN=ZNF609 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
EVGIPAPNAVATLPDNIK
01817.9887 
EVGIPAPNAVATLPDNIK
light3+607.00351817.9887150.0000 
FVTPVPGPQGK
01125.6182 
FVTPVPGPQGK
light2+563.81641125.6182150.0000 
DTSKPTPGTSLFTPSEGAASK
02078.0168 
DTSKPTPGTSLFTPSEGAASK
light3+693.67952078.0168150.0000 
SGDGANAGGLVAAIAPK
01467.7682 
SGDGANAGGLVAAIAPK
light2+734.89141467.7682150.0000 
SEGVGTCSEK
01052.4444 
SEGVGTCSEK
light2+527.22951052.4444100.0000 
SEGVGTCSEKDPGVLQPVPLGGR
12338.1587 
SEGVGTCSEKDPGVLQPVPLGGR
light3+780.39352338.1587150.0000 
DPGVLQPVPLGGR
01303.7248 
DPGVLQPVPLGGR
light2+652.86971303.7248150.0000 
TYVGTLLDCTR
01297.6336 
TYVGTLLDCTR
light2+649.82411297.6336140.0000 
FCDSPTSDLEMR
01456.5963 
FCDSPTSDLEMR
light2+729.30541456.5963150.0000 
MRPNSNTPVNETATASDSK
02018.9327 
MRPNSNTPVNETATASDSK
light3+673.98492018.9327150.0000 
GSQNSSEHRPPASSTSEDVK
02098.9516 
GSQNSSEHRPPASSTSEDVK
light3+700.65782098.9516150.0000 
NKPLSDMELNSSSEDSK
01879.8469 
NKPLSDMELNSSSEDSK
light3+627.62291879.8469150.0000 
TNSMGSATGPLPGTK
01417.6871 
TNSMGSATGPLPGTK
light2+709.85081417.6871150.0000 
NCPSPVLIDCPHPNCNKK
12148.9867 
NCPSPVLIDCPHPNCNKK
light3+717.33622148.9867150.0000 
ESSKELESPLTPGK
11500.7672 
ESSKELESPLTPGK
light2+751.39091500.7672150.0000 
ESSKELESPLTPGK
11500.7672 
ESSKELESPLTPGK
light3+501.26301500.7672140.0000 
ELESPLTPGK
01069.5655 
ELESPLTPGK
light2+535.79001069.5655130.0000 
MEGLLNGSSDPHQSR
01626.7420 
MEGLLNGSSDPHQSR
light2+814.37831626.7420150.0000 
MEGLLNGSSDPHQSR
01626.7420 
MEGLLNGSSDPHQSR
light3+543.25461626.7420150.0000 
AEADKIYSFTDNAPSPSIGGSSR
12369.1135 
AEADKIYSFTDNAPSPSIGGSSR
light3+790.71182369.1135150.0000 
IYSFTDNAPSPSIGGSSR
01854.8748 
IYSFTDNAPSPSIGGSSR
light3+619.29891854.8748150.0000 
TNSPAYSDISDAGEDGEGKVDSVK
12440.0878 
TNSPAYSDISDAGEDGEGKVDSVK
light3+814.36992440.0878150.0000 
SKDAEQLVK
11016.5502 
SKDAEQLVK
light2+509.28241016.5502150.0000 
RDEEPESIEGK
11287.5943 
RDEEPESIEGK
light2+644.80441287.5943140.0000 
DEEPESIEGK
01131.4932 
DEEPESIEGK
light2+566.75391131.4932100.0000 
VKNDICEEK
11133.5387 
VKNDICEEK
light2+567.77661133.5387140.0000 
QQYEEQQKR
11235.5895 
QQYEEQQKR
light2+618.80201235.5895120.0000 
QSLEQQQR
01015.5047 
QSLEQQQR
light2+508.75961015.5047120.0000 
APSLTDLVK
0942.5386 
APSLTDLVK
light2+472.2766942.5386130.0000 
AKEPGADPAK
1982.5084 
AKEPGADPAK
light2+492.2615982.5084110.0000 
LDDSSKLPGQAPEGLK
11653.8574 
LDDSSKLPGQAPEGLK
light2+827.93601653.8574150.0000 
LDDSSKLPGQAPEGLK
11653.8574 
LDDSSKLPGQAPEGLK
light3+552.29311653.8574150.0000 
LPGQAPEGLK
01008.5604 
LPGQAPEGLK
light2+505.28751008.5604150.0000 
LSDASHLSK
0956.4927 
LSDASHLSK
light2+479.2536956.4927150.0000 
QAEMDPILWYR
01420.6809 
QAEMDPILWYR
light2+711.34771420.6809130.0000 
MWTYVYPAK
01157.5580 
MWTYVYPAK
light2+579.78631157.558090.0000 
YSDIKSEDER
11240.5572 
YSDIKSEDER
light2+621.28591240.5572150.0000 
YSDIKSEDER
11240.5572 
YSDIKSEDER
light3+414.52631240.5572120.0000 
ESTSSDCKLPTSEESR
11811.7843 
ESTSSDCKLPTSEESR
light3+604.93541811.7843140.0000 
LPTSEESR
0917.4454 
LPTSEESR
light2+459.7300917.4454100.0000 
VSGGEDADKAR
11103.5207 
VSGGEDADKAR
light2+552.76761103.5207150.0000 
ALDILQQHASHYK
01522.7892 
ALDILQQHASHYK
light2+762.40191522.7892150.0000 
ALDILQQHASHYK
01522.7892 
ALDILQQHASHYK
light3+508.60371522.7892150.0000 
DRGGCGVVGGGGSCSSVGGASGGER
12237.9502 
DRGGCGVVGGGGSCSSVGGASGGER
light3+746.99072237.9502150.0000 
GGCGVVGGGGSCSSVGGASGGER
01966.8221 
GGCGVVGGGGSCSSVGGASGGER
light3+656.61471966.8221150.0000 
sp|O15194|CTDSL_HUMANCTD small phosphatase-like protein OS=Homo sapiens OX=9606 GN=CTDSPL PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
YLLPEVTVLDYGK
01508.8126 
YLLPEVTVLDYGK
light2+755.41361508.8126140.0000 
YLLPEVTVLDYGKK
11636.9076 
YLLPEVTVLDYGKK
light3+546.64311636.9076150.0000 
RPHVDEFLQR
01295.6735 
RPHVDEFLQR
light3+432.89841295.6735150.0000 
YADPVADLLDR
01246.6194 
YADPVADLLDR
light2+624.31701246.6194150.0000 
sp|O43159|RRP8_HUMANRibosomal RNA-processing protein 8 OS=Homo sapiens OX=9606 GN=RRP8 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
RQLLATLR
1969.6083 
RQLLATLR
light2+485.8114969.6083130.0000 
KASFASASAEVGK
11251.6459 
KASFASASAEVGK
light2+626.83021251.6459150.0000 
KASFASASAEVGK
11251.6459 
KASFASASAEVGK
light3+418.22261251.6459150.0000 
ASFASASAEVGK
01123.5510 
ASFASASAEVGK
light2+562.78281123.5510150.0000 
ASFASASAEVGKK
11251.6459 
ASFASASAEVGKK
light2+626.83021251.6459150.0000 
ASFASASAEVGKK
11251.6459 
ASFASASAEVGKK
light3+418.22261251.6459150.0000 
QGPPCSDSEEEVER
01617.6577 
QGPPCSDSEEEVER
light2+809.83611617.6577150.0000 
QALVGSDSAEDEK
01347.6154 
QALVGSDSAEDEK
light2+674.81501347.6154150.0000 
QALVGSDSAEDEKR
11503.7165 
QALVGSDSAEDEKR
light2+752.86551503.7165150.0000 
QALVGSDSAEDEKR
11503.7165 
QALVGSDSAEDEKR
light3+502.24611503.7165150.0000 
HAPINSAQHLDNVDQTGPK
02040.9977 
HAPINSAQHLDNVDQTGPK
light3+681.33982040.9977150.0000 
GSTTNDPPK
0915.4298 
GSTTNDPPK
light2+458.7222915.4298100.0000 
QSPGSTSPKPPHTLSR
01675.8642 
QSPGSTSPKPPHTLSR
light2+838.93941675.864290.0000 
QSPGSTSPKPPHTLSR
01675.8642 
QSPGSTSPKPPHTLSR
light3+559.62871675.8642150.0000 
FQPPQVPDQAPAEAPTEK
01948.9531 
FQPPQVPDQAPAEAPTEK
light3+650.65831948.9531150.0000 
TEVSPVPR
0883.4763 
TEVSPVPR
light2+442.7454883.4763110.0000 
YLNEQLYSGPSSAAQR
01782.8537 
YLNEQLYSGPSSAAQR
light2+892.43411782.8537150.0000 
YLNEQLYSGPSSAAQR
01782.8537 
YLNEQLYSGPSSAAQR
light3+595.29181782.8537150.0000 
LFQEDPEAFLLYHR
01776.8835 
LFQEDPEAFLLYHR
light2+889.44901776.8835150.0000 
LFQEDPEAFLLYHR
01776.8835 
LFQEDPEAFLLYHR
light3+593.30181776.8835150.0000 
WPLQPVDR
01009.5345 
WPLQPVDR
light2+505.77451009.5345110.0000 
QRPASLVVADFGCGDCR
01906.8778 
QRPASLVVADFGCGDCR
light3+636.63321906.8778150.0000 
NPVHCFDLASLDPR
01639.7777 
NPVHCFDLASLDPR
light2+820.89611639.7777150.0000 
NPVHCFDLASLDPR
01639.7777 
NPVHCFDLASLDPR
light3+547.59981639.7777150.0000 
VLKPGGLLK
0923.6168 
VLKPGGLLK
light2+462.8157923.6168120.0000 
DLTNSHFFLFDFQK
01757.8413 
DLTNSHFFLFDFQK
light2+879.92791757.8413150.0000 
DLTNSHFFLFDFQK
01757.8413 
DLTNSHFFLFDFQK
light3+586.95441757.8413150.0000 
TGPPLVGPK
0864.5069 
TGPPLVGPK
light2+433.2607864.5069120.0000 
AQLSGLQLQPCLYK
01617.8549 
AQLSGLQLQPCLYK
light2+809.93471617.8549150.0000 
AQLSGLQLQPCLYK
01617.8549 
AQLSGLQLQPCLYK
light3+540.29221617.8549150.0000 
sp|O75056|SDC3_HUMANSyndecan-3 OS=Homo sapiens OX=9606 GN=SDC3 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
GARPGPGLLDNAIDSGSSAAQLPQK
02419.2456 
GARPGPGLLDNAIDSGSSAAQLPQK
light3+807.42252419.2456150.0000 
sp|O94875|SRBS2_HUMANSorbin and SH3 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=SORBS2 PE=1 SV=3
Peptide Note/Annotations
Precursor Note/Annotations
APHYPGIGPVDESGIPTAIR
02046.0534 
APHYPGIGPVDESGIPTAIR
light3+683.02512046.0534150.0000 
SSTEKHDWDPPDR
11568.6855 
SSTEKHDWDPPDR
light3+523.90251568.6855150.0000 
SSILQHERPASLYQSSIDR
02186.1080 
SSILQHERPASLYQSSIDR
light3+729.70992186.1080150.0000 
VDLPGSSTTLTK
01217.6503 
VDLPGSSTTLTK
light2+609.83241217.6503150.0000 
SFTSSSPSSPSR
01225.5575 
SFTSSSPSSPSR
light2+613.78601225.5575130.0000 
SYSSTLTDMGR
01216.5394 
SYSSTLTDMGR
light2+609.27701216.5394100.0000 
VGIFPISYVEK
01250.6911 
VGIFPISYVEK
light2+626.35281250.6911140.0000 
AQPARPPPPAQPGEIGEAIAK
02094.1222 
AQPARPPPPAQPGEIGEAIAK
light3+699.04802094.1222150.0000 
sp|O95620|DUS4L_HUMANtRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Homo sapiens OX=9606 GN=DUS4L PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
SDCMQTTICQER
01527.6116 
SDCMQTTICQER
light2+764.81311527.6116130.0000 
WAMAEGYGACLINKPELVQDMVK
02622.2644 
WAMAEGYGACLINKPELVQDMVK
light3+875.09542622.2644150.0000 
NQVETPGFSVSIK
01404.7249 
NQVETPGFSVSIK
light2+703.36971404.7249150.0000 
AEATGVSWITVHGR
01482.7579 
AEATGVSWITVHGR
light3+495.25991482.7579150.0000 
ENMSIPVIANGDIR
01527.7715 
ENMSIPVIANGDIR
light2+764.89301527.7715150.0000 
ITGTDGVMVAR
01118.5754 
ITGTDGVMVAR
light2+560.29501118.5754150.0000