sp|P09151|LEU1_ECOLI: /JBEI/T.S. Batth et al. Metab. Eng. 2014 - A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins/Individual QconCAT Methods

sp|P09151|LEU1_ECOLI

Individual QconCAT Methods

Protein

File	Qcat1-Ind.sky 132 KB SkyP file (link only, 1KB) Full Skyline file Copy relative URL to clipboard Copy full URL to clipboard
Description	2-isopropylmalate synthase OS=Escherichia coli (strain K12) GN=leuA PE=1 SV=5
Decoy	false
Note

Sequence Coverage

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By: Replicate: Modified forms: Combined Stacked

The number displayed on each peptide box is the rank
1										11										21										31										41										51										61										71										81										91
M	S	Q	Q	V	I	I	F	D	T	T	L	R	D	G	E	Q	A	L	Q	A	S	L	S	V	K	E	K	L	Q	I	A	L	A	L	E	R	M	G	V	D	V	M	E	V	G	F	P	V	S	S	P	G	D	F	E	S	V	Q	T	I	A	R	Q	V	K	N	S	R	V	C	A	L	A	R	C	V	E	K	D	I	D	V	A	A	E	S	L	K	V	A	E	A	F	R	I	H	T	F	I
																																																																																									1

101										111										121										131										141										151										161										171										181										191
A	T	S	P	M	H	I	A	T	K	L	R	S	T	L	D	E	V	I	E	R	A	I	Y	M	V	K	R	A	R	N	Y	T	D	D	V	E	F	S	C	E	D	A	G	R	T	P	I	A	D	L	A	R	V	V	E	A	A	I	N	A	G	A	T	T	I	N	I	P	D	T	V	G	Y	T	M	P	F	E	F	A	G	I	I	S	G	L	Y	E	R	V	P	N	I	D	K	A	I	I	S
																																													2

201										211										221										231										241										251										261										271										281										291
V	H	T	H	D	D	L	G	L	A	V	G	N	S	L	A	A	V	H	A	G	A	R	Q	V	E	G	A	M	N	G	I	G	E	R	A	G	N	C	S	L	E	E	V	I	M	A	I	K	V	R	K	D	I	L	N	V	H	T	A	I	N	H	Q	E	I	W	R	T	S	Q	L	V	S	Q	I	C	N	M	P	I	P	A	N	K	A	I	V	G	S	G	A	F	A	H	S	S	G	I	H


301										311										321										331										341										351										361										371										381										391
Q	D	G	V	L	K	N	R	E	N	Y	E	I	M	T	P	E	S	I	G	L	N	Q	I	Q	L	N	L	T	S	R	S	G	R	A	A	V	K	H	R	M	D	E	M	G	Y	K	E	S	E	Y	N	L	D	N	L	Y	D	A	F	L	K	L	A	D	K	K	G	Q	V	F	D	Y	D	L	E	A	L	A	F	I	G	K	Q	Q	E	E	P	E	H	F	R	L	D	Y	F	S	V	Q	S


401										411										421										431										441										451										461										471										481										491
G	S	N	D	I	A	T	A	A	V	K	L	A	C	G	E	E	V	K	A	E	A	A	N	G	N	G	P	V	D	A	V	Y	Q	A	I	N	R	I	T	E	Y	N	V	E	L	V	K	Y	S	L	T	A	K	G	H	G	K	D	A	L	G	Q	V	D	I	V	A	N	Y	N	G	R	R	F	H	G	V	G	L	A	T	D	I	V	E	S	S	A	K	A	M	V	H	V	L	N	N	I	W


501										511										521
R	A	A	E	V	E	K	E	L	Q	R	K	A	Q	H	N	E	N	N	K	E	T	V

Intensity

(Log 10 base)

Annotations for sp|P09151|LEU1_ECOLI

Description	2-isopropylmalate synthase
Gene name	leuA
Organisms	Escherichia coli Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12)

Genbank IDs	GIs	Swiss-Prot Accessions	Swiss-Prot Names	Ensembl	IPI Numbers	GO Categories	Biological Processes	Molecular Functions
YP_002925272.1 NP_414616.1 YP_488380.1	none loaded	P09151 P78044 C4ZPZ7	LEU1_ECOLI LEU1_ECOBW	none loaded	none loaded	GO:0003852 GO:0003985 GO:0009098	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis	Transferase

Peptides

Select All Select None Show Selected Show Unselected Show All	Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Avg. Measured RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Predicted RT Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	RT Score Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
	VAEAFR	691.3653	0	1.3657
	TPIADLAR	855.4814	0	5.7842

Chromatograms

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Summary Charts

Peak Areas

Retention Times