Aducanumab_mini_assay_variability_2025-02-26_14-38-16.sky.zip: /Indiana U - Proteome Analysis/Reproducability and repeatability Aducanumab Assay/Reproducibility and Repeatability

Aducanumab_mini_assay_variability_2025-02-26_14-38-16.sky.zip

Reproducibility and Repeatability

Document Summary

Aducanumab_mini_assay_variability_2025-02-26_14-38-16.sky.zip 1 version

4 proteins, 4 peptides, 8 precursors, 512 transitions - 30 replicates - Skyline (64-bit) 21.2.0.568 (17c47d8e1)

Precursor List

Protein Note/Annotations

sp|P0001|Aducanumab

Chimeric Light

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
LLIYAASSLQSGVPSR	Adu_light2	0	1660.9148		LLIYAASSLQSGVPSR		light	2+	831.4647	1660.9148	59	0.0000
LLIYAASSLQSGVPSR	Adu_light2	0	1660.9148		LLIYAASSLQSGVPSR		heavy	2+	836.4688	1670.9231	59	0.0000

sp|P0000|Aducanumab

Chimeric Heavy

Adu_heavy

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
AEDTAVYYCAR	Adu_heavy2	0	1317.5660		AEDTAVYYCAR		light	2+	659.7903	1317.5660	39	0.0000
AEDTAVYYCAR	Adu_heavy2	0	1317.5660		AEDTAVYYCAR		heavy	2+	664.7944	1327.5742	39	0.0000

sp|P0000|Aducanumab

Chimeric Heavy

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
LSCAASGFAFSSYGMHWVR	Adu_heavy1_MeOx	0	2148.9510		LSCAASGFAFSSYGMHWVR		light	3+	717.3243	2148.9510	96	0.0000
LSCAASGFAFSSYGMHWVR	Adu_heavy1_MeOx	0	2148.9510		LSCAASGFAFSSYGMHWVR		heavy	3+	720.6604	2158.9592	96	0.0000

sp|P0001|Aducanumab

Chimeric Light

Peptide Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Peptide Note/Annotations	Missed Cleavages Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Peptide Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Rank Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Precursor Note/Annotations	Label Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Bar Chart Pie Chart Quick Chart	Q1 Z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Q1 m/z Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Precursor Neutral Mass Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	Transition Count Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	CE Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart	DP Sort Ascending Sort Descending Clear Sort Filter... Clear Filter Remove Column Summary Statistics... Box & Whisker Quick Chart
DIQMTQSPSSLSASVGDR	Adu_light1_MeOx	0	1893.8738		DIQMTQSPSSLSASVGDR		light	2+	947.9442	1893.8738	62	0.0000
DIQMTQSPSSLSASVGDR	Adu_light1_MeOx	0	1893.8738		DIQMTQSPSSLSASVGDR		heavy	2+	952.9483	1903.8821	62	0.0000