082618_iMRM_mAb-Plex2_Exp1_CalCurve_upload_v3_noblanks_2018-08-26_12-20-15.sky.zip

ResponseCurve
082618_iMRM_mAb-Plex2_Exp1_CalCurve_upload_v3_noblanks_2018-08-26_12-20-15.sky.zip   1 version
 
19 proteins, 46 peptides, 98 precursors, 412 transitions  -  30 replicates  -  Skyline (64-bit) 4.1.0.11796
Protein Note/Annotations
 
sp|P31749|AKT1_HUMAN RAC-alpha serine/threonine-protein kinase OS=Homo sapiens GN=AKT1 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
SLLSGLLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
0829.5273 SLLSGLLKlight2+415.7709829.5273316.0103 
SLLSGLLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
0829.5273 SLLSGLLKheavy2+419.7780837.5415316.0103 
FFAGIVWQHVYEKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01622.8245 FFAGIVWQHVYEKlight2+812.41961622.8245428.7428 
FFAGIVWQHVYEKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01622.8245 FFAGIVWQHVYEKheavy2+816.42661630.8387428.7428 
FFAGIVWQHVYEKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01622.8245 FFAGIVWQHVYEKlight3+541.94881622.8245318.5115 
FFAGIVWQHVYEKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01622.8245 FFAGIVWQHVYEKheavy3+544.62021630.8387318.5115 
sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kinase OS=Homo sapiens GN=AKT2 PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
LLPPFKPQVTSEVDTRPeptideConcentration: 1200
PeptideConcentrationIS: 120
01825.9938 LLPPFKPQVTSEVDTRlight3+609.67191825.9938519.9878 
LLPPFKPQVTSEVDTRPeptideConcentration: 1200
PeptideConcentrationIS: 120
01825.9938 LLPPFKPQVTSEVDTRheavy3+613.00801836.0021519.9878 
sp|Q9Y243|AKT3_HUMAN RAC-gamma serine/threonine-protein kinase OS=Homo sapiens GN=AKT3 PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
DEVAHTLTESRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01256.5997 DEVAHTLTESRlight2+629.30711256.5997422.8649 
DEVAHTLTESRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01256.5997 DEVAHTLTESRheavy2+634.31131266.6080422.8649 
EGITDAATMKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01035.4907 EGITDAATMKlight2+518.75261035.4907419.3161 
EGITDAATMKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01035.4907 EGITDAATMKheavy2+522.75971043.5049419.3161 
sp|P10398|ARAF_HUMAN Serine/threonine-protein kinase A-Raf OS=Homo sapiens GN=ARAF PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
GLNQDCCVVYRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01382.6071 GLNQDCCVVYRlight2+692.31081382.6071424.8873 
GLNQDCCVVYRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01382.6071 GLNQDCCVVYRheavy2+697.31501392.6154424.8873 
IGTGSFGTVFRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01140.5928 IGTGSFGTVFRlight2+571.30371140.5928421.0029 
IGTGSFGTVFRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01140.5928 IGTGSFGTVFRheavy2+576.30781150.6010421.0029 
TQADELPACLLSAARPeptideConcentration: 4000
PeptideConcentrationIS: 40
01614.8036 TQADELPACLLSAARlight2+808.40911614.8036428.6140 
TQADELPACLLSAARPeptideConcentration: 4000
PeptideConcentrationIS: 40
01614.8036 TQADELPACLLSAARheavy2+813.41321624.8118428.6140 
sp|P15056|BRAF_HUMAN Serine/threonine-protein kinase B-raf OS=Homo sapiens GN=BRAF PE=1 SV=4
Peptide Note/Annotations
Precursor Note/Annotations
GLIPECCAVYRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01336.6268 GLIPECCAVYRlight2+669.32071336.6268424.1493 
GLIPECCAVYRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01336.6268 GLIPECCAVYRheavy2+674.32481346.6350424.1493 
sp|P24385|CCND1_HUMAN G1/S-specific cyclin-D1 OS=Homo sapiens GN=CCND1 PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
AYPDANLLNDRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01260.6099 AYPDANLLNDRlight2+631.31221260.6099522.9292 
AYPDANLLNDRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01260.6099 AYPDANLLNDRheavy2+636.31631270.6181522.9292 
AEETCAPSVSYFKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01487.6602 AEETCAPSVSYFKlight2+744.83741487.6602526.5734 
AEETCAPSVSYFKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01487.6602 AEETCAPSVSYFKheavy2+748.84451495.6744526.5734 
FLSLEPVKKPeptideConcentration: 4400
PeptideConcentrationIS: 440
11059.6328 FLSLEPVKKlight3+354.21821059.6328414.4190 
FLSLEPVKKPeptideConcentration: 4400
PeptideConcentrationIS: 440
11059.6328 FLSLEPVKKheavy3+356.88961067.6470414.4190 
VIKCDPDCLRPeptideConcentration: 4000
PeptideConcentrationIS: 40
11274.6111 VIKCDPDCLRlight2+638.31281274.6111323.1539 
VIKCDPDCLRPeptideConcentration: 4000
PeptideConcentrationIS: 40
11274.6111 VIKCDPDCLRheavy2+643.31701284.6194323.1539 
VIKCDPDCLRPeptideConcentration: 4000
PeptideConcentrationIS: 40
11274.6111 VIKCDPDCLRlight3+425.87771274.6111415.9811 
VIKCDPDCLRPeptideConcentration: 4000
PeptideConcentrationIS: 40
11274.6111 VIKCDPDCLRheavy3+429.21371284.6194415.9811 
ACQEQIEALLESSLRPeptideConcentration: 1200
PeptideConcentrationIS: 120
01745.8618 ACQEQIEALLESSLRlight3+582.96121745.8618519.4056 
ACQEQIEALLESSLRPeptideConcentration: 1200
PeptideConcentrationIS: 120
01745.8618 ACQEQIEALLESSLRheavy3+586.29731755.8701519.4056 
sp|P12830|CADH1_HUMAN Cadherin-1 OS=Homo sapiens GN=CDH1 PE=1 SV=3
Peptide Note/Annotations
Precursor Note/Annotations
GQVPENEANVVITTLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01710.9152 GQVPENEANVVITTLKlight2+856.46491710.9152530.1566 
GQVPENEANVVITTLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01710.9152 GQVPENEANVVITTLKheavy2+860.47201718.9294530.1566 
GLDARPEVTRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01112.5938 GLDARPEVTRlight3+371.87191112.5938314.8038 
GLDARPEVTRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01112.5938 GLDARPEVTRheavy3+375.20801122.6021314.8038 
sp|P19022|CADH2_HUMAN Cadherin-2 OS=Homo sapiens GN=CDH2 PE=1 SV=4
Peptide Note/Annotations
Precursor Note/Annotations
LSDPANWLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01042.5447 LSDPANWLKlight2+522.27961042.5447419.4293 
LSDPANWLKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01042.5447 LSDPANWLKheavy2+526.28671050.5589419.4293 
SAAPHPGDIGDFINEGLKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01836.9006 SAAPHPGDIGDFINEGLKlight3+613.30751836.9006520.0671 
SAAPHPGDIGDFINEGLKPeptideConcentration: 4400
PeptideConcentrationIS: 440
01836.9006 SAAPHPGDIGDFINEGLKheavy3+615.97891844.9148520.0671 
sp|P00533|EGFR_HUMAN Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2
Peptide Note/Annotations
Precursor Note/Annotations
IPLENLQIIRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01207.7289 IPLENLQIIRlight2+604.87171207.7289522.0805 
IPLENLQIIRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01207.7289 IPLENLQIIRheavy2+609.87581217.7371522.0805 
NLQEILHGAVRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01248.6939 NLQEILHGAVRlight2+625.35421248.6939322.7380 
NLQEILHGAVRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01248.6939 NLQEILHGAVRheavy2+630.35831258.7021322.7380 
NLQEILHGAVRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01248.6939 NLQEILHGAVRlight3+417.23861248.6939315.7928 
NLQEILHGAVRPeptideConcentration: 4000
PeptideConcentrationIS: 40
01248.6939 NLQEILHGAVRheavy3+420.57471258.7021315.7928 
sp|P04626|ERBB2_HUMAN Receptor tyrosine-protein kinase erbB-2 OS=Homo sapiens GN=ERBB2 PE=1 SV=1
Peptide Note/Annotations
Precursor Note/Annotations
GIWIPDGENVKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01226.6295 GIWIPDGENVKlight2+614.32201226.6295522.3838 
GIWIPDGENVKPeptideConcentration: 4000
PeptideConcentrationIS: 40
01226.6295 GIWIPDGENVKheavy2+618.32911234.6437522.3838