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Targeted MS Runs
061918_iMRM_AllPhos_IMAC_CalCurve_JKedit_annotated2_jw2.sky (1).zip
ResponseCurve
Document Summary
061918_iMRM_AllPhos_IMAC_CalCurve_JKedit_annotated2_jw2.sky (1).zip
17 MB
SkyP file (link only, 1KB)
Full Skyline file
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1 version
35 proteins
,
49 peptides
,
102 precursors
,
456 transitions
-
29 replicates
- Skyline 20.2
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sp|O43524|FOXO3_HUMAN
Forkhead box protein O3 OS=Homo sapiens GN=FOXO3 PE=1 SV=1
Peptide
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AV
S
MDNSNK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1044.3947
AV
S
MDNSNK
light
2+
523.2046
1044.3947
4
19.4590
AV
S
MDNSNK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1044.3947
AV
S
MDNSN
K
heavy
2+
527.2117
1052.4089
4
19.4590
AV
S
MDNSNKYTK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
1436.6007
AV
S
MDNSNKYTK
light
2+
719.3076
1436.6007
3
25.7539
AV
S
MDNSNKYTK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
1436.6007
AV
S
MDNSNKYT
K
heavy
2+
723.3147
1444.6149
3
25.7539
sp|P00533|EGFR_HUMAN
Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2
Peptide
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Y
SSDPTGALTEDSIDDTFLPVPEYINQSVPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
3477.5753
Y
SSDPTGALTEDSIDDTFLPVPEYINQSVPK
light
3+
1160.1990
3477.5753
5
31.9893
Y
SSDPTGALTEDSIDDTFLPVPEYINQSVPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
3477.5753
Y
SSDPTGALTEDSIDDTFLPVPEYINQSVP
K
heavy
3+
1162.8704
3485.5895
5
31.9893
GSHQISLDNPD
Y
QQDFFPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2314.9896
GSHQISLDNPD
Y
QQDFFPK
light
3+
772.6705
2314.9896
5
23.5412
GSHQISLDNPD
Y
QQDFFPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2314.9896
GSHQISLDNPD
Y
QQDFFP
K
heavy
3+
775.3419
2323.0038
5
23.5412
GSTAENAE
Y
LR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1289.5289
GSTAENAE
Y
LR
light
2+
645.7717
1289.5289
5
23.3934
GSTAENAE
Y
LR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1289.5289
GSTAENAE
Y
L
R
heavy
2+
650.7759
1299.5372
5
23.3934
sp|P00533|EGFR_HUMAN
Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2
Peptide
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YSSDPTGALTEDSIDDTFLPVPE
Y
INQSVPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
3477.5753
YSSDPTGALTEDSIDDTFLPVPE
Y
INQSVPK
light
3+
1160.1990
3477.5753
5
31.9893
YSSDPTGALTEDSIDDTFLPVPE
Y
INQSVPK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
3477.5753
YSSDPTGALTEDSIDDTFLPVPE
Y
INQSVP
K
heavy
3+
1162.8704
3485.5895
5
31.9893
sp|P04049|RAF1_HUMAN
RAF proto-oncogene serine/threonine-protein kinase OS=Homo sapiens GN=RAF1 PE=1 SV=1
Peptide
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ST
S
TPNVHMVSTTLPVDSR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2107.9609
ST
S
TPNVHMVSTTLPVDSR
light
3+
703.6609
2107.9609
5
22.0368
ST
S
TPNVHMVSTTLPVDSR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2107.9609
ST
S
TPNVHMVSTTLPVDS
R
heavy
3+
706.9970
2117.9692
5
22.0368
sp|P04626|ERBB2_HUMAN
Receptor tyrosine-protein kinase erbB-2 OS=Homo sapiens GN=ERBB2 PE=1 SV=1
Peptide
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LLDIDETE
Y
HADGGK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1754.7400
LLDIDETE
Y
HADGGK
light
3+
585.9206
1754.7400
3
19.4701
LLDIDETE
Y
HADGGK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1754.7400
LLDIDETE
Y
HADGG
K
heavy
3+
588.5920
1762.7542
3
19.4701
GTPTAENPE
Y
LGLDVPV
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1850.8339
GTPTAENPE
Y
LGLDVPV
light
2+
926.4242
1850.8339
5
32.4023
GTPTAENPE
Y
LGLDVPV
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
1850.8339
GTPTAENPE
Y
LGLDVP
V
heavy
2+
929.4311
1856.8477
5
32.4023
sp|P10398|ARAF_HUMAN
Serine/threonine-protein kinase A-Raf OS=Homo sapiens GN=ARAF PE=1 SV=2
Peptide
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NLGYRD
S
GYYWEVPPSEVQLLK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
2692.2574
NLGYRD
S
GYYWEVPPSEVQLLK
light
3+
898.4264
2692.2574
4
26.2827
NLGYRD
S
GYYWEVPPSEVQLLK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
2692.2574
NLGYRD
S
GYYWEVPPSEVQLL
K
heavy
3+
901.0978
2700.2716
4
26.2827
D
S
GYYWEVPPSEVQLLK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2088.9445
D
S
GYYWEVPPSEVQLLK
light
2+
1045.4795
2088.9445
4
36.2240
D
S
GYYWEVPPSEVQLLK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2088.9445
D
S
GYYWEVPPSEVQLL
K
heavy
2+
1049.4866
2096.9587
4
36.2240
sp|P15056|BRAF_HUMAN
Serine/threonine-protein kinase B-raf OS=Homo sapiens GN=BRAF PE=1 SV=4
Peptide
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GDGGSTTGLSA
T
PPASLPGSLTNVK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2364.1210
GDGGSTTGLSA
T
PPASLPGSLTNVK
light
3+
789.0476
2364.1210
4
23.8982
GDGGSTTGLSA
T
PPASLPGSLTNVK
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2364.1210
GDGGSTTGLSA
T
PPASLPGSLTNV
K
heavy
3+
791.7190
2372.1352
4
23.8982
sp|P15056|BRAF_HUMAN
Serine/threonine-protein kinase B-raf OS=Homo sapiens GN=BRAF PE=1 SV=4
Peptide
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RD
S
SDDWEIPDGQITVGQR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
2252.9699
RD
S
SDDWEIPDGQITVGQR
light
3+
751.9972
2252.9699
4
23.0905
RD
S
SDDWEIPDGQITVGQR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
1
2252.9699
RD
S
SDDWEIPDGQITVGQ
R
heavy
3+
755.3333
2262.9782
4
23.0905
D
S
SDDWEIPDGQITVGQR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2096.8688
D
S
SDDWEIPDGQITVGQR
light
2+
1049.4417
2096.8688
5
36.3512
D
S
SDDWEIPDGQITVGQR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2096.8688
D
S
SDDWEIPDGQITVGQ
R
heavy
2+
1054.4458
2106.8771
5
36.3512
sp|P27361|MK03_HUMAN
Mitogen-activated protein kinase 3 OS=Homo sapiens GN=MAPK3 PE=1 SV=4
Peptide
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Precursor
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CE
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DP
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IADPEHDHTGFL
T
E
Y
VATR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2330.9610
IADPEHDHTGFL
T
E
Y
VATR
light
3+
777.9943
2330.9610
5
23.6573
IADPEHDHTGFL
T
E
Y
VATR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2330.9610
IADPEHDHTGFL
T
E
Y
VAT
R
heavy
3+
781.3304
2340.9693
5
23.6573
sp|P27361|MK03_HUMAN
Mitogen-activated protein kinase 3 OS=Homo sapiens GN=MAPK3 PE=1 SV=4
Peptide
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CE
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DP
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IADPEHDHTGFLTE
Y
VATR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2250.9947
IADPEHDHTGFLTE
Y
VATR
light
3+
751.3388
2250.9947
5
23.0762
IADPEHDHTGFLTE
Y
VATR
PeptideConcentration: 2000
PeptideConcentrationIS: 100
0
2250.9947
IADPEHDHTGFLTE
Y
VAT
R
heavy
3+
754.6749
2261.0029
5
23.0762