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Targeted MS Runs
DQ180414_iMRM_mAb-Plex2_Exp1_CalCurve_v1_jw.sky.zip
ChromatogramLibrary
Document Summary
DQ180414_iMRM_mAb-Plex2_Exp1_CalCurve_v1_jw.sky.zip
496 KB
SkyP file (link only, 1KB)
Full Skyline file
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1 version
19 proteins
,
44 peptides
,
92 precursors
,
384 transitions
-
1 replicate
- Skyline 20.2
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sp|P00533|EGFR_HUMAN
Epidermal growth factor receptor OS=Homo sapiens GN=EGFR PE=1 SV=2
Peptide
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IPLENLQIIR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1207.7289
IPLENLQIIR
light
2+
604.8717
1207.7289
5
22.0805
IPLENLQIIR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1207.7289
IPLENLQII
R
heavy
2+
609.8758
1217.7371
5
22.0805
NLQEILHGAVR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1248.6939
NLQEILHGAVR
light
3+
417.2386
1248.6939
3
15.7928
NLQEILHGAVR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1248.6939
NLQEILHGAV
R
heavy
3+
420.5747
1258.7021
3
15.7928
sp|P01100|FOS_HUMAN
Proto-oncogene c-Fos OS=Homo sapiens GN=FOS PE=1 SV=1
Peptide
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APHPFGVPAPSAGAYSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1680.8372
APHPFGVPAPSAGAYSR
light
3+
561.2864
1680.8372
4
18.9330
APHPFGVPAPSAGAYSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1680.8372
APHPFGVPAPSAGAYS
R
heavy
3+
564.6224
1690.8455
4
18.9330
TEPFDDFLFPASSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1627.7518
TEPFDDFLFPASSR
light
2+
814.8832
1627.7518
4
28.8219
TEPFDDFLFPASSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1627.7518
TEPFDDFLFPASS
R
heavy
2+
819.8873
1637.7601
4
28.8219
GSSSNEPSSDSLSSPTLLAL
PeptideConcentration: 1200
PeptideConcentrationIS: 120
0
1947.9273
GSSSNEPSSDSLSSPTLLAL
light
2+
974.9709
1947.9273
4
33.9607
GSSSNEPSSDSLSSPTLLAL
PeptideConcentration: 1200
PeptideConcentrationIS: 120
0
1947.9273
GSSSNEPSSDSLSSPTLLA
L
heavy
2+
978.4795
1954.9445
4
33.9607
sp|P04049|RAF1_HUMAN
RAF proto-oncogene serine/threonine-protein kinase OS=Homo sapiens GN=RAF1 PE=1 SV=1
Peptide
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GLQPE
C
C
AVFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1335.6064
GLQPE
C
C
AVFR
light
2+
668.8105
1335.6064
4
24.1329
GLQPE
C
C
AVFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1335.6064
GLQPE
C
C
AVF
R
heavy
2+
673.8146
1345.6146
4
24.1329
VVDPTPEQFQAFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1532.7623
VVDPTPEQFQAFR
light
2+
767.3884
1532.7623
4
27.2973
VVDPTPEQFQAFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1532.7623
VVDPTPEQFQAF
R
heavy
2+
772.3926
1542.7706
4
27.2973
GYASPDLSK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
936.4553
GYASPDLSK
light
2+
469.2349
936.4553
4
17.7265
GYASPDLSK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
936.4553
GYASPDLS
K
heavy
2+
473.2420
944.4695
4
17.7265
sp|P04626|ERBB2_HUMAN
Receptor tyrosine-protein kinase erbB-2 OS=Homo sapiens GN=ERBB2 PE=1 SV=1
Peptide
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GIWIPDGENVK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1226.6295
GIWIPDGENVK
light
2+
614.3220
1226.6295
5
22.3838
GIWIPDGENVK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1226.6295
GIWIPDGENV
K
heavy
2+
618.3291
1234.6437
5
22.3838
sp|P10398|ARAF_HUMAN
Serine/threonine-protein kinase A-Raf OS=Homo sapiens GN=ARAF PE=1 SV=2
Peptide
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GLNQD
C
C
VVYR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1382.6071
GLNQD
C
C
VVYR
light
2+
692.3108
1382.6071
4
24.8873
GLNQD
C
C
VVYR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1382.6071
GLNQD
C
C
VVY
R
heavy
2+
697.3150
1392.6154
4
24.8873
IGTGSFGTVFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1140.5928
IGTGSFGTVFR
light
2+
571.3037
1140.5928
4
21.0029
IGTGSFGTVFR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1140.5928
IGTGSFGTVF
R
heavy
2+
576.3078
1150.6010
4
21.0029
TQADELPA
C
LLSAAR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1614.8036
TQADELPA
C
LLSAAR
light
2+
808.4091
1614.8036
4
28.6140
TQADELPA
C
LLSAAR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1614.8036
TQADELPA
C
LLSAA
R
heavy
2+
813.4132
1624.8118
4
28.6140
sp|P12830|CADH1_HUMAN
Cadherin-1 OS=Homo sapiens GN=CDH1 PE=1 SV=3
Peptide
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GQVPENEANVVITTLK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1710.9152
GQVPENEANVVITTLK
light
2+
856.4649
1710.9152
4
30.1566
GQVPENEANVVITTLK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1710.9152
GQVPENEANVVITTL
K
heavy
2+
860.4720
1718.9294
4
30.1566
GLDARPEVTR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1112.5938
GLDARPEVTR
light
3+
371.8719
1112.5938
3
14.8038
GLDARPEVTR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1112.5938
GLDARPEVT
R
heavy
3+
375.2080
1122.6021
3
14.8038
sp|P15056|BRAF_HUMAN
Serine/threonine-protein kinase B-raf OS=Homo sapiens GN=BRAF PE=1 SV=4
Peptide
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GLIPE
C
C
AVYR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1336.6268
GLIPE
C
C
AVYR
light
2+
669.3207
1336.6268
4
24.1493
GLIPE
C
C
AVYR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1336.6268
GLIPE
C
C
AVY
R
heavy
2+
674.3248
1346.6350
4
24.1493
sp|P19022|CADH2_HUMAN
Cadherin-2 OS=Homo sapiens GN=CDH2 PE=1 SV=4
Peptide
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LSDPANWLK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1042.5447
LSDPANWLK
light
2+
522.2796
1042.5447
4
19.4293
LSDPANWLK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1042.5447
LSDPANWL
K
heavy
2+
526.2867
1050.5589
4
19.4293
SAAPHPGDIGDFINEGLK
PeptideConcentration: 4400
PeptideConcentrationIS: 440
0
1836.9006
SAAPHPGDIGDFINEGLK
light
3+
613.3075
1836.9006
5
20.0671
SAAPHPGDIGDFINEGLK
PeptideConcentration: 4400
PeptideConcentrationIS: 440
0
1836.9006
SAAPHPGDIGDFINEGL
K
heavy
3+
615.9789
1844.9148
5
20.0671
sp|P21860|ERBB3_HUMAN
Receptor tyrosine-protein kinase erbB-3 OS=Homo sapiens GN=ERBB3 PE=1 SV=1
Peptide
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CE
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DP
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LTFQLEPNPHTK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1423.7460
LTFQLEPNPHTK
light
3+
475.5893
1423.7460
4
17.0648
LTFQLEPNPHTK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1423.7460
LTFQLEPNPHT
K
heavy
3+
478.2607
1431.7602
4
17.0648
YLERGESIEPLDPSEK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1860.9105
YLERGESIEPLDPSEK
light
3+
621.3108
1860.9105
5
20.2416
YLERGESIEPLDPSEK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1860.9105
YLERGESIEPLDPSE
K
heavy
3+
623.9822
1868.9247
5
20.2416
SLEATDSAFDNPDYWHSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
2109.9028
SLEATDSAFDNPDYWHSR
light
3+
704.3082
2109.9028
5
22.0509
SLEATDSAFDNPDYWHSR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
2109.9028
SLEATDSAFDNPDYWHS
R
heavy
3+
707.6443
2119.9111
5
22.0509
sp|P24385|CCND1_HUMAN
G1/S-specific cyclin-D1 OS=Homo sapiens GN=CCND1 PE=1 SV=1
Peptide
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CE
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DP
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AYPDANLLNDR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1260.6099
AYPDANLLNDR
light
2+
631.3122
1260.6099
4
22.9292
AYPDANLLNDR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1260.6099
AYPDANLLND
R
heavy
2+
636.3163
1270.6181
4
22.9292
AEET
C
APSVSYFK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1487.6602
AEET
C
APSVSYFK
light
2+
744.8374
1487.6602
5
26.5734
AEET
C
APSVSYFK
PeptideConcentration: 4000
PeptideConcentrationIS: 40
0
1487.6602
AEET
C
APSVSYF
K
heavy
2+
748.8445
1495.6744
5
26.5734
FLSLEPVKK
PeptideConcentration: 4400
PeptideConcentrationIS: 440
1
1059.6328
FLSLEPVKK
light
3+
354.2182
1059.6328
4
14.4190
FLSLEPVKK
PeptideConcentration: 4400
PeptideConcentrationIS: 440
1
1059.6328
FLSLEPVK
K
heavy
3+
356.8896
1067.6470
4
14.4190
VIK
C
DPD
C
LR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1274.6111
VIK
C
DPD
C
LR
light
2+
638.3128
1274.6111
3
23.1539
VIK
C
DPD
C
LR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1274.6111
VIK
C
DPD
C
L
R
heavy
2+
643.3170
1284.6194
3
23.1539
VIK
C
DPD
C
LR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1274.6111
VIK
C
DPD
C
LR
light
3+
425.8777
1274.6111
4
15.9811
VIK
C
DPD
C
LR
PeptideConcentration: 4000
PeptideConcentrationIS: 40
1
1274.6111
VIK
C
DPD
C
L
R
heavy
3+
429.2137
1284.6194
4
15.9811
A
C
QEQIEALLESSLR
PeptideConcentration: 1200
PeptideConcentrationIS: 120
0
1745.8618
A
C
QEQIEALLESSLR
light
3+
582.9612
1745.8618
5
19.4056
A
C
QEQIEALLESSLR
PeptideConcentration: 1200
PeptideConcentrationIS: 120
0
1745.8618
A
C
QEQIEALLESSL
R
heavy
3+
586.2973
1755.8701
5
19.4056